Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Apparatus and methods for preparing tissue grafts

A tissue graft, graft technology, applied in biochemical equipment and methods, methods of supporting/fixing microorganisms, tissue culture, etc., can solve problems such as coronary applicability limitations

Inactive Publication Date: 2008-09-03
特苏鲁尼斯公司
View PDF11 Cites 22 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, despite the above advances, clinical coronary applicability has been limited to date because catheters do not maintain a sufficiently viscous non-thrombogenic surface, so studies have focused on additional in vitro maturation times

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Apparatus and methods for preparing tissue grafts
  • Apparatus and methods for preparing tissue grafts
  • Apparatus and methods for preparing tissue grafts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0038] The present invention provides means for preparing various tissue implants or grafts by applying pressure, preferably sustained low magnitude pressure, to adhere or "plan" cells to any suitable graft scaffold or other permeable matrix material and methods. In specific embodiments, the tissue is tubular tissue, such as vascular tissue. However, the invention is also applicable to any type of tissue graft that involves the adhesion of cells to a scaffold or other matrix material, including but not limited to skin, cartilage, bone, bone marrow, tendon, ligament, gastrointestinal , genitourinary tract, liver, pancreas, kidney, adrenal gland, mucosal epithelium, and nerve grafts. The method is particularly well suited for tubular tissues including, but not limited to, those of the cardiovascular system and urinary system.

[0039] As used herein, the term "sustained low-amplitude pressure" refers to pressure with a pressure head of about 10 mmHg, about 15 mmHg, about 20 mm...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides automated apparatus and methods for manufacturing implantable tissue grafts in an operating room setting, preferably utilizing autologous adipose tissue cells for preparing vascular grafts. The automated apparatus includes media and tissue dissociating chemical reservoirs, filters, a cell separator and a perfusion flow loop through a biochamber which supports a graft substrate. The present invention further provides methods for using the tissue grafts and cell samples prepared by the devices described herein in a multitude of therapies including revascularization, regeneration and reconstruction of tissues and organs, as well as treatment and prevention of diseases. The invention also provides methods biochamber configurations for applying a sustained low pressure gradient across a permeable scaffold material using a media containing cells to be deposited on the scaffold for the production of tissue grafts, including, but not limited to, vascular grafts.

Description

[0001] This application claims priority to U.S. Patent Application Serial No. 60 / 697,954, filed July 12, 2005, and U.S. Patent Application Serial No. 11 / 314,281, filed December 22, 2005, both of which are Incorporated herein by reference. [0002] A statement about federally funded research or development [0003] This invention was made with Government support under Grant Numbers #W81XWH-04-1-0503 and #W81XWH-05-1-0620 awarded by the United States Army Medical Research and Material Command . technical field [0004] The present invention relates to tissue engineering systems involving mammalian cells and cell products for use in the manufacture of tissue implants and grafts, including but not limited to artificial blood vessels and arteriovenous grafts. In particular, the present invention relates to methods and methods for adhering (adhering) adipose-derived mesenchymal cells or other cells to a permeable scaffold material by sustained pressure cell sodding (sustained pres...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00A61F2/08
CPCC12M25/14C12M29/10C12M45/06C12M21/08
Inventor 斯图尔特·K·威廉姆斯保罗·科斯尼科克里斯·英格兰托马斯·F·坎农埃里克·福斯曼尤金·博兰克里斯蒂安·L·哈勒克雷格·A·毛赫
Owner 特苏鲁尼斯公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com