Apparatus and methods for preparing tissue grafts

A tissue graft, graft technology, applied in biochemical equipment and methods, methods of supporting/fixing microorganisms, tissue culture, etc., can solve problems such as coronary applicability limitations

Inactive Publication Date: 2008-09-03
特苏鲁尼斯公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, despite the above advances, clinical coronary applicability has been limited to date because catheters do not maintain a sufficiently viscous non-thrombogenic surface, so studies have focused on additional in vitro maturation times

Method used

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  • Apparatus and methods for preparing tissue grafts
  • Apparatus and methods for preparing tissue grafts
  • Apparatus and methods for preparing tissue grafts

Examples

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Embodiment Construction

[0038] The present invention provides means for preparing various tissue implants or grafts by applying pressure, preferably sustained low magnitude pressure, to adhere or "plan" cells to any suitable graft scaffold or other permeable matrix material and methods. In specific embodiments, the tissue is tubular tissue, such as vascular tissue. However, the invention is also applicable to any type of tissue graft that involves the adhesion of cells to a scaffold or other matrix material, including but not limited to skin, cartilage, bone, bone marrow, tendon, ligament, gastrointestinal , genitourinary tract, liver, pancreas, kidney, adrenal gland, mucosal epithelium, and nerve grafts. The method is particularly well suited for tubular tissues including, but not limited to, those of the cardiovascular system and urinary system.

[0039] As used herein, the term "sustained low-amplitude pressure" refers to pressure with a pressure head of about 10 mmHg, about 15 mmHg, about 20 mm...

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PUM

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Abstract

The invention provides automated apparatus and methods for manufacturing implantable tissue grafts in an operating room setting, preferably utilizing autologous adipose tissue cells for preparing vascular grafts. The automated apparatus includes media and tissue dissociating chemical reservoirs, filters, a cell separator and a perfusion flow loop through a biochamber which supports a graft substrate. The present invention further provides methods for using the tissue grafts and cell samples prepared by the devices described herein in a multitude of therapies including revascularization, regeneration and reconstruction of tissues and organs, as well as treatment and prevention of diseases. The invention also provides methods biochamber configurations for applying a sustained low pressure gradient across a permeable scaffold material using a media containing cells to be deposited on the scaffold for the production of tissue grafts, including, but not limited to, vascular grafts.

Description

[0001] This application claims priority to U.S. Patent Application Serial No. 60 / 697,954, filed July 12, 2005, and U.S. Patent Application Serial No. 11 / 314,281, filed December 22, 2005, both of which are Incorporated herein by reference. [0002] A statement about federally funded research or development [0003] This invention was made with Government support under Grant Numbers #W81XWH-04-1-0503 and #W81XWH-05-1-0620 awarded by the United States Army Medical Research and Material Command . technical field [0004] The present invention relates to tissue engineering systems involving mammalian cells and cell products for use in the manufacture of tissue implants and grafts, including but not limited to artificial blood vessels and arteriovenous grafts. In particular, the present invention relates to methods and methods for adhering (adhering) adipose-derived mesenchymal cells or other cells to a permeable scaffold material by sustained pressure cell sodding (sustained pres...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00A61F2/08
CPCC12M25/14C12M29/10C12M45/06C12M21/08
Inventor 斯图尔特·K·威廉姆斯保罗·科斯尼科克里斯·英格兰托马斯·F·坎农埃里克·福斯曼尤金·博兰克里斯蒂安·L·哈勒克雷格·A·毛赫
Owner 特苏鲁尼斯公司
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