Supercharge Your Innovation With Domain-Expert AI Agents!

Method for the quantitative determination of poloxamers

A poloxamer, quantitative determination technology, applied in the field of liquid protein samples, can solve quantitative detection and other problems

Active Publication Date: 2008-09-10
ARES TRADING SA
View PDF10 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Currently there is no way to quantitatively detect poloxamers in protein samples because the molecular weight of the protein is comparable to that of poloxamers

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for the quantitative determination of poloxamers
  • Method for the quantitative determination of poloxamers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0208] Embodiment 1 (with reference to accompanying drawing 1)

[0209] The purpose of this example is to analyze the commercially available liquid hCG preparation Ovitrelle TM The concentration of Poloxamer 188 in its samples after 18 months storage at room temperature, and thus the stability of the liquid formulation was evaluated with respect to Poloxamer 188. Poloxamer 188 was prepared at a concentration of approximately 100 mcg / ml. Ovitrelle TM The quantitative detection scheme of poloxamer 188 is as follows:

[0210] Liquid Injectable Ovitrelle TM Contains the following ingredients: Chorionic Gonadotropin Alpha, Mannitol, L-Methionine, Poloxamer 188, Phosphoric Acid, NaOH and Water.

[0211] 1. Equipment and materials

[0212] supplier

[0213] HPLC ALLIANCE Model 269 Waters (Waters)

[0214] RI Detector Model 2414 Waters

[0215] Software Empower Waters

[0216] Personal computer IBM (or equivalent

[0217] ...

Embodiment 2

[0281] The purpose of this example is to analyze the commercially available liquid hFSH preparation Gonal-F RFF Pen TM Poloxamer 188 concentrations in their samples after 18 months storage at room temperature, and thus the stability of the liquid formulations was assessed with respect to Poloxamer 188. Poloxamer 188 was prepared at a concentration of approximately 100 mcg / ml. In addition to the column flow rate of 0.75ml / min, Gonal-F RFF Pen TM The quantification protocol for poloxamer 188 was the same as shown in Example 1 for Ovitrelle.

[0282] Poloxamers can be separated from hFSH and quantified separately.

Embodiment 3

[0284] The purpose of this example is to analyze the commercially available liquid hGH preparation Serostim TM Poloxamer 188 concentration in samples. Scheme is identical with embodiment 2.

[0285] Poloxamers can be separated from hGH and quantified separately.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention relates to the analytical determination of poloxamers in a liquid protein sample.

Description

technical field [0001] The invention relates to the field of analysis and detection of surfactants belonging to poloxamers in liquid protein samples. Background of the invention [0002] Poloxamers are nonionic block copolymers of ethylene oxide (EO) and propylene oxide (PO). They are used as surfactants, emulsifiers, solubilizers and dispersants in pharmaceutical formulations. [0003] A well-known analytical method for the identification of poloxamers is calorimetry, which measures the UV absorption at 320 and 620 nm of the complex formed by poloxamers and cobalt(II) thiocyanate. [0004] Yun Mao et al. (Journal of Pharmaceutical and Biomedical Analysis, 35(2004), 1127) described molecular sieve chromatography (SEC) (using a chromatographic column with THF as mobile phase) and refractive index (RI) analysis in poloxamer detection applications. This approach has been used in pharmaceutical formulations Avapro, gabapentin (Neurontin) and pseudoephedrine hydrochloride (Sud...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): B01D15/34G01N33/487G01N33/68
CPCB01D15/34B01J20/28004B01J20/28083G01N30/02G01N33/487G01N33/68G01N33/6866G01N33/74G01N33/76B01D15/426
Inventor M·罗西
Owner ARES TRADING SA
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com