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HCV IgM antibody rapid detection test paper

A hepatitis C virus, detection test strip technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effects of high sensitivity, strong specificity, and rapid response

Inactive Publication Date: 2008-09-17
天津中新科炬生物制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is no report on detection of HCV IgM by immunochromatographic colloidal gold technique

Method used

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  • HCV IgM antibody rapid detection test paper

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Experimental program
Comparison scheme
Effect test

Embodiment

[0021] Embodiment: Preparation of HCV IgM antibody rapid detection test paper

[0022] 1 main material

[0023] 1.1 HCV CORE, NS3 fusion antigen: American Biotech Atlantic Inc (BAI.) product, used for colloidal gold labeling; mouse anti-human IgM, anti-HCV antibody: product of Academy of Military Medical Sciences, used for NC membrane coating; chloroauric acid: Products of Sigma Company; nitrocellulose (NC) membrane: products of Millipore Company; bovine serum albumin (BSA), polyethylene glycol (PEG) 20000, hydrolyzed casein: products of Sigma. Other commonly used reagents are analytical reagents.

[0024] 1.2 Clinical sera were obtained from relevant hospitals or blood banks by the company, 80 HCV IgG antibody positive sera and 300 HCV antibody negative sera.

[0025] 1.3 HCV IgM antibody detection ELISA kit, product of Abbott, USA.

[0026] 2 methods

[0027] 2.1 Preparation of colloidal gold-labeled chloroauric acid-trisodium citrate reduction method for HCV recombinant...

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PUM

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Abstract

The invention relates to a biology applied technique field, especially relates to a hepatitis C virus antibody quick detection test paper, comprising a sampling pad (1); a colloidal gold pad (2) closely connected with one end of the sampling pad and containing HCV labelled mixed antigen; a cellulose nitrate (NC) membrane closely connected with another end of the sampling pad and a sucking sample pad (4) closely connected with another end of the NC membrane; a test T line (5) and quality control C line (6) mutually separated with each other and coated by NC membrane, wherein the T line is anti human IgM antibody coated on the NC membrane and the C line is anti HCV antibody coated on the NC membrane. During detecting, the detected sample is added on the sampling pad of the test paper and the immunoreaction result is directly observed to accomplish detection. The invention has features: high sensitivity, specificity, quickspeed, convenience and is suitable for detection on site and real time recording and storing the result.

Description

technical field [0001] The invention relates to the technical field of biological applications, in particular to a rapid detection test paper for the rapid detection of hepatitis C virus (HCV) IgM antibody by colloidal gold immunochromatography. Background technique [0002] HCV was first discovered in 1989 by American Choo et al. from infected chimpanzee blood specimens. It is mainly transmitted by blood and body fluids, accounting for 70% of post-transfusion hepatitis. According to the World Health Organization, an estimated 170 million people worldwide are infected with HCV. The chronicity rate of hepatitis C is 50%-85%, and 20% of them can develop into liver fibrosis, which is very harmful. There are about 40 million HCV antibody carriers in my country. So far there is neither an effective vaccine nor an effective treatment for hepatitis C, and prevention of infection is the only effective way. HCV antibody inspection, hepatitis B surface antigen (HBsAg), human immuno...

Claims

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Application Information

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IPC IPC(8): G01N33/576G01N33/558G01N33/532
Inventor 李洲杨发青
Owner 天津中新科炬生物制药股份有限公司
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