Cultivation method for Dendrobium initial species embryo

A technology of embryo culture and dendrobium capsules, which is applied in the field of orchid plant cultivation, can solve the problems of uneven seedlings, slow propagation of seedlings, and large differences between plants, so as to achieve neat seedlings, fast reproduction, and differences among plants. small effect

Inactive Publication Date: 2008-09-24
INST OF FORESTRY CHINESE ACAD OF FORESTRY
View PDF0 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There are at least the following disadvantages in the above-mentioned prior art: the dependence on the mother plant is strong, and the num

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] The method for cultivating the original seed embryo of Dendrobium orchid of the present invention, its preferred embodiment is, at first, collect the dendrobium orchid capsule that seed embryo has matured, fruit pod is uncracked, carry out surface disinfection treatment; Then, cut pericarp and take out seed embryo ; Afterwards, the seed embryos were implanted in sterilized medium for culture.

[0015] When carrying out surface disinfection treatment, the Dendrobium orchid capsules can be scrubbed with about 70% ethanol for 1 to 2 minutes, then soaked in about 10% calcium hypochlorite supernatant for about 20 minutes, and then rinsed with sterile water for 2 to 4 minutes. times or so.

[0016] After cutting the pericarp and taking out the seed embryo, there are two methods for implanting the seed embryo into the sterilized medium:

[0017] One is to immerse the seed embryo in sterile water and shake it evenly, use a sterile straw to suck out the seed embryo with water, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a dendrobe orchid initial embryo breeding method. First, a dendrobe orchid capsule of which the embryo is ripe and the seed pod is indehiscent is collected, washed by 70 percent of ethanol for 1min-2min, then is dipped by 10 percent of supernatant fluid of calcium hypochlorite for 20min, and then washed by sterile water for twice to four times; then the embryo is taken out by cutting the fruit skin; finally the embryo is planted in the sterilized culture medium for culturing. The embryo can be dipped in the sterile water for shaking up, the embryo with water is aspirated by a sterile sucker and evenly dripped into the culture medium in the microsphere way, and the embryo can also be evenly splashed on the surface of the culture medium in a powder shape. The sterile culture medium is adopted to perform sterile insemination to the embryo of the dendrobe orchid, the embryo bourgeons to form a stem and a bud, and then forms a completed plant through multiplication and rootage. The dendrobe orchid initial embryo breeding method has strong operability, simpleness and practicality, fast breeding, small difference among plants and regular seedling.

Description

technical field [0001] The invention relates to a method for cultivating orchids, in particular to a method for cultivating primary embryos of dendrobium orchids. Background technique [0002] Dendrobium (Dendrobium) is the second largest genus in the Orchidaceae (Orchidaceae), second only to Dendrobium in the number of native species, and 81 species (subspecies) have been discovered so far. The global distribution ranges from India, Myanmar, China and Japan in the north to Indonesia, Papua New Guinea, Australia and New Zealand in the south, the Malay Islands and the Philippine Islands. Dendrobium has high medicinal value and ornamental value. As a medicinal material, Dendrobium orchid has medicinal properties such as promoting body fluid, relieving cough, and moistening throat. As an ornamental Dendrobium, it is both beautiful and fragrant, and is a world-renowned flower. [0003] Dendrobium orchids are epiphytic on alpine rocks and trees. In recent years, due to forest ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00C12N5/04
Inventor 李振坚王雁缪崑
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap