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Technique for purifying spherosinin from leguminosae pointvetch or milk vetch

A technology of swainsonine and Astragalus, which is applied in the field of purifying swainsonine, can solve the problems of high extraction cost, long extraction cycle, and low purity, and achieve the effects of low extraction cost, shortened extraction cycle, and reduced pollution

Inactive Publication Date: 2008-09-24
杨凌天力生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0021] The invention provides a process for purifying swainsonine from plants of the leguminous Oxytropis or Astragalus, aiming at the problems in the prior art that the extraction period is long, the environment is easily polluted, the extraction cost is high and the purity is relatively low flaws and deficiencies

Method used

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  • Technique for purifying spherosinin from leguminosae pointvetch or milk vetch
  • Technique for purifying spherosinin from leguminosae pointvetch or milk vetch
  • Technique for purifying spherosinin from leguminosae pointvetch or milk vetch

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Effect test

Embodiment 1

[0037] Embodiment 1: Extraction of swainsonine in mutated Astragalus membranaceus

[0038] 1. Ultrasonic extraction of pure water is used to obtain water extract of large polar organic components

[0039] Weigh 1 kg of varietal astragalus plant hay powder, add 6000 mL of pure water at a ratio of 1:6 (g / ml), set up an ultrasonic circulation extractor, conduct ultrasonic extraction at 60°C and 20KHz for 0.5 hours each time, and collect the water extract. Add pure water, ultrasonically extract 3 times under the same conditions, and collect the water extract. Combine the water extracts, concentrate the water extracts to a density of 1.2, and obtain 200 mL of a concentrated water extract of large polar organic components. Take water to extract the concentrated solution, add 95% industrial alcohol at a ratio of 1:2.5 (v / v) for alcohol precipitation, filter to remove the insoluble part, and recover ethanol from the obtained filtrate to obtain 100 mL of water concentrated solution, w...

Embodiment 2

[0044] Embodiment 2: Extraction of swainsonine in mutated Astragalus membranaceus

[0045] 1. Ultrasonic extraction of pure water is used to obtain water extract of large polar organic components

[0046] Weigh 2 kg of varietal astragalus plant hay powder, add 16000 mL of pure water at a ratio of 1:8 (g / ml), set up an ultrasonic circulation extractor, conduct ultrasonic extraction at 60° C. and 20 KHz for 1 hour each time, and collect the water extract. Add pure water, ultrasonically extract 3 times under the same conditions, and collect the water extract. Combine the water extracts, concentrate the water extracts to a density of 1.3, and obtain 300 mL of a concentrated water extract of large polar organic components. Take water to extract the concentrated solution, add 95% industrial alcohol in a ratio of 1:3 (v / v) for alcohol precipitation, filter to remove the insoluble part, and recover ethanol from the obtained filtrate to obtain 150 mL of water concentrated solution, wh...

Embodiment 3

[0051] Embodiment 3: Extraction of swainsonine in Oxytropis gansu

[0052] 1. Ultrasonic extraction of pure water is used to obtain water extract of large polar organic components

[0053] Weigh 2 kg of Oxytropis gansu plant hay powder, add 16000 mL of pure water at a ratio of 1:8 (g / ml), set up an ultrasonic circulation extractor, conduct ultrasonic extraction at 60°C and 20KHz for 1 hour each time, and collect the water extract. Add pure water, ultrasonically extract 3 times under the same conditions, and collect the water extract. Combine the water extracts, concentrate the water extracts to a density of 1.3, and obtain 300 mL of a concentrated water extract of large polar organic components. Take water to extract the concentrated solution, add 95% industrial alcohol at a ratio of 1:3 (v / v) for alcohol precipitation, and filter to remove the insoluble part. The resulting filtrate recovered ethanol to obtain 150 mL of water concentrate, which was stored for future use.

...

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Abstract

The present invention belongs to the technical field of phyto-extraction processes, in particular to a process for purifying swainsonine from plants belonging to the oxytropis or astragalus of leguminosae. Aimed at the defects of the prior art, which include a long period of extraction, environmental pollution, high extraction cost and low purity, the technical scheme put forward by the present invention is a process for purifying swainsonine from plants belonging to the oxytropis and astragalus of leguminosae orderly includes the following steps: (1) Maximum-polarity organic component aqueous extract is obtained by ultrasonic pure water extraction; (2) Crude swainsonine extract is separated out by polar macroporous adsorption resin; (3) After step-by-step sublimations, a pure swainsonine product is produced. Compared with the prior proprietary technology, the present invention has the following advantages: (1) short extraction period; (2) environmental pollution reduction; (3) low extraction cost; (4) increased extraction rate and purity; (5) strong practicability.

Description

Technical field: [0001] The invention belongs to the technical field of plant extraction technology, and in particular relates to a technology for purifying swainsonine from plants of the genus Oxytropis or Astragalus. Background technique: [0002] (1) Pharmacological effects of swainsonine [0003] Swainsonine is an indolizidine alkaloid, which is a very strong competitive inhibitor of α-mannosidase, which can inhibit the expression of glycoproteins on the surface of tumor cells, thereby inhibiting the growth and metastasis of tumor cells At the same time, it can also stimulate the body's immune system, improve the body's immune function, promote the proliferation of immune cells, and enhance the ability to kill tumor cells. Because swainsonine has good anti-tumor activity and immune enhancement effect, it has attracted extensive attention of domestic scholars and has been used as a backup drug for anti-tumor drug screening. At present, the research and development of sw...

Claims

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Application Information

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IPC IPC(8): C07D471/04A61K36/48
Inventor 赵宝玉万雪攀刘忠艳
Owner 杨凌天力生物技术有限公司
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