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Anti-CD86 humanized monoclonal antibody

A monoclonal antibody and humanized technology, applied in the field of biological immunology, can solve the problems of weak tumor cell killing ability, short half-life of mouse antibody, weak ADCC effect, etc.

Active Publication Date: 2008-10-01
威海市蓝色经济研究院有限公司
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Problems solved by technology

With the continuous development of technology, mouse-derived monoclonal antibodies will gradually be replaced by humanized antibodies. The killing ability of cells is weak; it has weak affinity with Fc receptors on the surface of immune cells such as NK, and the ADCC effect mediated is weak; the half-life of mouse antibody in human blood circulation is short, and the time for it to exert ADCC and CDC effects is relatively short; Mouse monoclonal antibodies are immunogenic, and the host is prone to produce anti-antibodies and cause allergic reactions

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  • Anti-CD86 humanized monoclonal antibody
  • Anti-CD86 humanized monoclonal antibody
  • Anti-CD86 humanized monoclonal antibody

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Embodiment Construction

[0021] Using DNA recombination technology to insert the light chain and heavy chain variable region genes of the mouse monoclonal antibody into the expression vector containing the human antibody constant region, transform mammalian cells to express the human-mouse chimeric antibody, and the humanization degree reaches about 70%. , the variable region of the heterologous monoclonal antibody is completely retained, its affinity activity is maintained to the greatest extent, and the immunogenicity is reduced.

[0022] 1. Cloning of CD86 gene

[0023] Human blood was extracted from a vein, centrifuged by FICOLL density, and the nucleated cell part of the upper layer was taken. After washing with PBS, the total RNA was extracted by the Trizol method, and then cDNA was synthesized under the action of reverse transcriptase with Oligo(dT) as a primer, and then PCR was used. method to amplify the full-length gene of human CD86.

[0024] 2. Antigen preparation and animal immunization ...

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Abstract

The invention provides a CD86 humanized monoclonal antibody, an associated gene order and an expression system thereof. The monoclonal antibody provided by the invention greatly reduces the immunogenicity of the monoclonal antibody of a mouse, realizes the high expression thereof of serum free medium in engineering cells (such as CHO cell) and has expression products with high biological activity and can be applied to autoimmune diseases and preparing medicines that restrict transplanted organs from experiencing host rejection reaction.

Description

technical field [0001] The invention belongs to the field of biological immunology, specifically, the invention relates to monoclonal antibody technology, antibody humanization technology and gene recombination technology. Background technique [0002] The human immune system is the most important defense barrier for the human body to resist foreign virus infection and maintain good health. For foreign bodies (viruses, transplanted organs, etc.) T cells and B cells. With the help of CD3 antigen on its surface, T cells recognize and bind the presented antigen through the T cell receptor (TCR) on its surface, which is the first signal for the activation of the body's immune response; then, CD86 on the surface of APC cells binds to T CD28 on the cell surface is actually the second signal for T cell activation. After T cells are activated, activated T cells are produced to destroy and kill virus-infected or heterologous somatic cells, and prevent virus replication and spread....

Claims

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Application Information

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IPC IPC(8): C07K16/28C12N15/13C12N15/63C12N1/21C12N1/19C12N5/10A61K39/395A61P37/02A61P37/06
Inventor 郑勇冯冬晓卢小兵
Owner 威海市蓝色经济研究院有限公司
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