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Method for preparing dimolecular modified nanometer probe as well as application thereof

A nano-probe and bimolecular technology, which is applied in the analysis of materials, material analysis through electromagnetic means, material inspection products, etc., to achieve the effect of high sensitivity, easy operation and convenient operation

Active Publication Date: 2008-10-08
NANJING UNIV
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

However, the method of using nanomaterials as carriers to encode biomolecules by carrying small molecules that are easy to detect and using mass spectrometry has not been reported yet.

Method used

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  • Method for preparing dimolecular modified nanometer probe as well as application thereof
  • Method for preparing dimolecular modified nanometer probe as well as application thereof
  • Method for preparing dimolecular modified nanometer probe as well as application thereof

Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0035] The following examples are provided to illustrate and not to limit the scope of the invention of the application.

[0036] 1. Preparation of bimolecularly modified nanoprobes. (see attached figure 1 )

[0037] The first step, the preparation of a gold nano solution of about 13nm

[0038] Accurately weigh 133.1 mg of chloroauric acid, add 320 ml of ultrapure water, heat, and reflux. Quickly add 32ml of an aqueous solution containing 368.6mg of sodium citrate, continue to reflux for 15 minutes, cool down to room temperature, and set aside.

[0039] The second step, preparation of bimolecularly modified gold nanoprobes

[0040] A), preparation of nanoprobe 1

[0041] Oligonucleotide 2: (5'HS(CH 2 ) 6 -A 10 ATC CTT ATC AAT ATT 3′) and the encoded molecule 4 ([S(CH 2 ) 11 (OCH 2 CH 2 ) 5 OH] 2 , molecular weight 846) modified gold nanoparticles

[0042] Dissolve the recognition molecule 1 OD oligonucleotide 2 in 200 μl ultrapure water, add it to 2 ml of the g...

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Abstract

The invention discloses a method of the preparation for bimolecular-decorated nano probe, wherein one molecular is a recognition molecular and the other is an encode molecular; the obtained bimolecular-decorated nano probe detects bio-molecules in the way of sandwich-type combination; the sandwich-type combination is composed of three components of different functions:(1)a detection probe to generate signals,(2)a capture probe connected to a solid-state support, (3)a target part, through adding which, the detection probe and the capture probe can form sandwich-type combination with the bio-molecules, the bio-molecules to be detected, the capture probe and the detection probe form sandwich-type structure, and a clear detection signal can be obtained through detection after washing and removing impurities; with the method of sandwich-type hybridization, the bio-molecules to be detected can recognize its nano-probe combination and separate the samples, then encodes the molecule with mass spectrometric detection, thus qualitatively and quantitatively detects the bio-molecules in the samples to be identified quickly.

Description

technical field [0001] The invention belongs to the field of biomolecular detection. Nanotechnology and molecular self-assembly technology are used to prepare nanoprobes carrying recognition molecules and coding molecules, and mass spectrometers are used to detect coding molecules, thereby rapidly qualitatively and quantitatively detecting and identifying biomolecules in samples. . Background technique [0002] Biomolecular detection refers to the detection of biological macromolecules such as nucleic acids, proteins and sugars. Take, for example, the field of molecular diagnostics. With the advent of the polymerase chain reaction (PCR) technology in the mid-1980s and the start of the Human Genome Project in the early 1990s, the initial completion of the Human Genome Project and the conduct of other omics studies have made it possible for disease diagnosis to be developed. A more mature subject - molecular diagnostics. At present, the content of molecular diagnosis has de...

Claims

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Application Information

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IPC IPC(8): G01N27/64G01N33/53
Inventor 黄乐群邱飞姜大炜丁一冰朱进
Owner NANJING UNIV
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