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Galactosyl temperature-responsive polymer hydrogel and preparation method thereof

A polymer hydrogel, temperature-sensitive technology, applied in the field of intelligent polymer materials, can solve the problems of low biocompatibility, insufficient hydrophilicity and biocompatibility, and achieve good biocompatibility , not easy to break, good identification effect

Inactive Publication Date: 2008-10-29
TIANJIN POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[Teruo Okano, NorikoYamada, MinakoOkuhara et al. Biomaterials, 16 (1995) 297-303 (Teruo Okano, NorikoYamada, MinakoOkuhara et al.Biomaterials 16 (1995) 297-303)] utilize polyisopropylacrylamide ( PNIPAAm) for cell culture studies, however, PNIPAAm is not very biocompatible when used alone
[0011] From the above analysis, it can be seen that although PNIPAAm has temperature sensitivity, it shows insufficient hydrophilicity and biocompatibility.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) 12g lactobionic acid (LA) and 3g ethylenediamine (NH 2 CH 2 CH 2 NH 2 ) were dissolved in 30ml of dimethyl sulfoxide (DMSO), and reacted for about 2 hours at a temperature of 70° C., the mixture solution was poured into chloroform for precipitation, and the precipitated product was vacuum-dried.

[0028] (2) The intermediate L-NH of galactose terminal amino 2 and acrylic acid (AAc) were dissolved in 20ml dimethyl sulfoxide (DMSO), L-NH 2 The mass ratio with acrylic acid (AAc) is 4:1, the solution mixing concentration is 0.2g / ml, and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide salt with a total concentration of 0.1wt% is added Acid acid (EDC.HCL) and N-hydroxysulfosuccinimide (Sulfo-NHS); vortex stirring at room temperature for 20h, precipitated with chloroform, and dried in vacuo to obtain the product GAC.

[0029] (3) Weigh GAC and monomer NIPAAm respectively according to the mass ratio GAC:NIPAAm=0.2:1, dissolve the two in deionized water to make a 0.05g / ml...

Embodiment 2

[0031] (1) 12g lactobionic acid (LA) and 2g ethylenediamine (NH 2 CH 2 CH 2 NH 2 ) were dissolved in 20ml of dimethyl sulfoxide (DMSO), reacted for about 2 hours at a temperature of 70°C, poured the mixture solution into chloroform for precipitation, and dried the precipitated product in vacuum.

[0032] (2) The intermediate L-NH of galactose terminal amino 2 and acrylic acid (AAc) were dissolved in 20ml dimethyl sulfoxide (DMSO), L-NH 2 The mass ratio with acrylic acid (AAc) is 6:1, the mixed concentration of the solution is 0.5g / ml, and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide salt with a total concentration of 0.05wt% is added Acid acid (EDC.HCL) and N-hydroxysulfosuccinimide (Sulfo-NHS); vortex stirring at room temperature for 30h, precipitated with chloroform, and dried in vacuo to obtain the product GAC.

[0033] (3) Weigh GAC and monomer NIPAAm respectively according to the mass ratio GAC:NIPAAm=0.5:1, dissolve the two in deionized water to make a 0.1g / ml mixe...

Embodiment 3

[0035] (1) 12g lactobionic acid (LA) and 3g ethylenediamine (NH 2 CH 2 CH 2 NH 2 ) were respectively dissolved in 15ml of dimethyl sulfoxide (DMSO), reacted for about 4 hours at a temperature of 80° C., poured the mixture solution into chloroform for precipitation, and the precipitated product was vacuum-dried.

[0036] (2) The intermediate L-NH of galactose terminal amino 2 and acrylic acid (AAc) were dissolved in 20ml dimethyl sulfoxide (DMSO), L-NH 2 The mass ratio to acrylic acid (AAc) is 6:1, the solution mixing concentration is 0.3g / ml, and the total concentration is 0.15wt% of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide salt Acid acid (EDC.HCL) and N-hydroxysulfosuccinimide (Sulfo-NHS); vortex stirring at room temperature for 25 hours, precipitated with chloroform, and dried in vacuo to obtain the product GAC.

[0037] (3) Weigh GAC and monomer NIPAAm respectively according to the mass ratio GAC:NIPAAm=0.8:1, dissolve the two in deionized water to make a 0.2g / ml ...

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Abstract

The invention discloses a galactosyl-based temperature-sensitive polymer hydrogel and a preparation method thereof. The hydrogel is a Poly (NIPAAm-co-GAC) copolymerized hydrogel. The preparation method comprises three steps of: (1) synthesizing an intermediate L-NH2 with an aminogalactose group; (2) synthesizing an intermediate monomer GAC with galactosyl group and double bonds; and (3) preparing the Poly (NIPAAm-co-GAC) copolymerized hydrogel by using the NIPAAm (N-isopropylacrylamide) and the intermediate monomer GAC. The hydrogel has good temperature response and biocompatibility, and has good identification effect on hepatocytes; and can be used as a matrix material in tissue engineering for promoting cell adhension and growth in cell culture, and the cells can be automatically detached due to the temperature sensitivity of the hydrogel.

Description

technical field [0001] The invention relates to an intelligent polymer material technology, in particular to a temperature-sensitive N-isopropylacrylamide (N-isopropylacrylamide, NIPAAm) and galactose-based monomer (GAC) copolymerized hydrogel and its preparation The method technology belongs to the field of functional polymer materials. Background technique [0002] The field of tissue engineering uses various methods of combining living cells with biomaterials and growth factors to develop substitutes to repair, maintain or maintain the function of tissues and organs. In engineered tissues, seed cells (cultured cells or stem cells) are often planted on bioactive degradable scaffolds that can provide mechanical and chemical signals to guide cell differentiation and assembly into three-dimensional tissues. [0003] Tissue engineering often uses hydrogels (ie, polymer networks containing hydrophilic groups) as cell release vehicles. The structure of the hydrogel is similar ...

Claims

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Application Information

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IPC IPC(8): C08F220/54C08F2/00
Inventor 陈莉肖飞邢蕊峰
Owner TIANJIN POLYTECHNIC UNIV
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