Aspergillus niger proline protein endopeptidase and preparation method thereof

A technology of Aspergillus niger proline and Aspergillus proline, which is applied in the field of genetic engineering and enzymology to achieve the effect of high efficiency and simple and feasible process

Inactive Publication Date: 2008-10-29
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

[0006] Aspergillus niger is a biosafety bacterium recognized by the US Food and Drug Administration. Extracting p

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  • Aspergillus niger proline protein endopeptidase and preparation method thereof
  • Aspergillus niger proline protein endopeptidase and preparation method thereof
  • Aspergillus niger proline protein endopeptidase and preparation method thereof

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[0034] The following examples will further illustrate the present invention; the following examples are illustrative and not restrictive, and the scope of protection of the present invention cannot be limited by the following examples.

[0035] The first thing that needs to be explained is:

[0036] In the present invention, the term "Aspergillus niger proline protein endopeptidase" refers to a polypeptide of SEQ ID No. 2 sequence having Aspergillus niger proline protein endopeptidase activity. The term also includes variant forms of the SEQ ID No. 2 sequence that have the same function as the Aspergillus niger proline protein endopeptidase. These variant forms include (but are not limited to): deletions and insertions of several (usually 1-50, preferably 1-30, more preferably 1-20, and most preferably 1-10) amino acids And / or substitution, and adding one or several (usually within 20, preferably within 10, more preferably within 5) amino acids at the C-terminal and / or N-terminal....

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Abstract

The invention relates to Aspergillus niger proline-specific endoprotease and the preparation method thereof, which belong to the field of genetic engineering and enzymology. The Aspergillus niger proline-specific endoprotease is selected from amino acid sequence of Aspergillus niger proline-specific endoprotease with a sequence code of SEQ ID No.2, wherein the amino acid sequence has nucleotide sequences represented by the first to the 1,581 in SEQ ID No.1, and has polypeptides of amino acid sequence represented by SEQ ID No.2. Test shows that haze protein bands with molecular weight of 8 to 14 kDa are disappeared completely after SDS-polyacrylamide gel electrophoresis detection of beer protein added with PEP, which shows that the haze activity protein rich in proline residues is hydrolyzed completely by PEP, and primarily testifies that proline-specific endoprotease can specifically cut protein and proline residues at carbon terminal of a peptide chain, and can eliminate cold haze substance in beer.

Description

technical field [0001] The invention relates to the fields of genetic engineering and enzymology, and more specifically relates to an Aspergillus niger proline endopeptidase and a preparation method thereof. Background technique [0002] Proline protein endopeptidase, prolyl endopeptidase, (prolyl-specific endoprotease, proline-specific endoprotease, proline-specific endopeptidase PEP for short) all refer to an enzyme in this invention. Proline endopeptidase is a serine protease that can specifically hydrolyze the peptide bond at the carboxy-terminal of proline in small molecular weight polypeptides (Wilk S. Life Science, 1983, 33(22): 2149). [0003] As we all know, among the 20 kinds of α-amino acids that make up natural proteins or polypeptides, proline (Pro) is the only amino acid with an imine structure, and its ring structure affects the spatial conformation when forming peptide bonds with other amino acids. Pro-containing peptide bonds or other adjacent peptide bonds...

Claims

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Application Information

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IPC IPC(8): C12N9/62C12N15/57C12N15/63
Inventor 路福平杨迪王海宽李玉黎明王春霞周丽娜王斌哲向先长
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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