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Anti-hypertensive casein active peptide and method for producing the same as well as use thereof

An anti-hypertensive and casein technology, applied in the fields of application, peptide/protein composition, protein food processing, etc., can solve the problem that it is difficult to determine anti-hypertensive active peptide products, and achieve easy operation, good effect, and convenient source Effect

Inactive Publication Date: 2008-12-24
BRIGHT DAIRY & FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most of the antihypertensive peptide products reported in the literature are ACE inhibitors (ACEI), but they have not been verified by animal experiments, so it is difficult to determine that they are real antihypertensive active peptide products

Method used

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  • Anti-hypertensive casein active peptide and method for producing the same as well as use thereof
  • Anti-hypertensive casein active peptide and method for producing the same as well as use thereof
  • Anti-hypertensive casein active peptide and method for producing the same as well as use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Enzyme reaction using enzyme reactor:

[0027] (1) Casein dissolution

[0028] Add 1000ml of water into the reactor, heat up to the optimum temperature of about 55°C, weigh 150g of casein (Shanghai Chemical Reagent Company), slowly and gradually put it into the water under magnetic stirring, adjust the pH to about 8.5 with 2mol / L NaOH, Under the condition of 55°C, all the casein was dissolved, so that the concentration of casein in the reactor was 15% (w / v, g / ml).

[0029] (2) Enzymolysis

[0030] According to the ratio of enzyme activity unit / substrate weight of 0.072AU / g casein, add 4.5g food-grade protease Alcalase (Novozymes Biotechnology Co., Ltd., 2.4L, enzyme activity 2.4AU / g), and maintain the reaction temperature at 55 ℃, and 2mol / L NaOH was added dropwise through the ZDJ-4A automatic potentiometric titrator to maintain the pH of the enzymolysis solution at about 8.5, and the enzymolysis time was 5 hours. The free amino nitrogen in the enzymolysis solutio...

Embodiment 2

[0038] Change the temperature in the above step 1(1) casein dissolution and step 1(2) enzymolysis to 60°C, pH to 8.6, substrate concentration to 12% (w / v, g / ml), and the rest to be implemented in the same way Example 1, preparing the antihypertensive peptide of the present invention.

Embodiment 3

[0040]Change the temperature during the above step 1(1) casein dissolution and step 1(2) enzymolysis to 58°C, the pH is 8.4, the substrate concentration is 18% (w / v, g / ml), and the rest are implemented in the same way Example 1, preparing the antihypertensive peptide of the present invention.

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Abstract

The invention discloses a method for producing bioactive peptide with a high blood pressure resistant function from casein, which comprises the steps of enzymolysis reaction and the desalting of zymolyte, wherein, zymolyte in the enzymolysis reaction is casein aqueous solution; prolease Alcalase is selected as enzyme; the consumption of the enzyme is 0.072 AU / g bioactive peptide; the temperature of the whole reaction process is controlled to be 55 DEG C to 60 DEG C; the pH value is between 8.4 to 8.6; the enzymolysis time is 5 to 6 hours. The invention also discloses the casein bioactive peptide prepared through the method. The bioactive peptide has good high blood pressure resistant function, and can be used to prepare high blood pressure resistant drinks, food or medicine.

Description

technical field [0001] The present invention relates to a casein active peptide, in particular to a method for producing an active peptide with antihypertensive function from casein, and the casein active peptide with antihypertensive function prepared by the method and its application . Background technique [0002] Animal milk, such as cow milk, is a biological fluid with complex components, the protein content of which is 32g / L, mainly composed of casein and whey protein, and casein accounts for about 80% (26g / L), which is containing A complex of several proteins of phosphorus, present in milk in colloidal form. Milk protein is a high-quality full-valent protein in nutrition, containing a variety of bioactive peptide fragments, which have many physiological functions. [0003] Angiotensin-converting enzyme (ACE) (EC3.4.15.1) is a multifunctional enzyme consisting of two peptide chains. ACE is a membrane-bound extrinsic metalloprotease mainly present in the vascular endo...

Claims

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Application Information

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IPC IPC(8): A23J3/10A23J3/34A23L1/305A61K38/00A61P9/12A23L33/18
Inventor 宫霞郭本恒王荫榆
Owner BRIGHT DAIRY & FOOD
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