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Method for detecting staphylococcus aureus enterotoxin by nanostructured composite metal

A Staphylococcus enterotoxin and nanostructure technology, which is applied in the measurement of scattering characteristics and phase influence characteristics, etc., can solve the problems of weak plasmon resonance intensity, insignificant signal response, inability to apply Staphylococcus aureus enterotoxin, etc., and achieve cost-effective long time effect

Inactive Publication Date: 2011-09-14
INST OF OPTICS & ELECTRONICS - CHINESE ACAD OF SCI
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  • Abstract
  • Description
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Problems solved by technology

[0004] At present, localized surface plasmon resonance cannot be applied to the detection of Staphylococcus aureus enterotoxin. Biomolecular membranes for efficient recognition of toxins
When the metal nanostructure material is gold, although an effective biomolecular film can be formed, the local surface plasmon resonance intensity is very weak, and the signal response is not obvious

Method used

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  • Method for detecting staphylococcus aureus enterotoxin by nanostructured composite metal
  • Method for detecting staphylococcus aureus enterotoxin by nanostructured composite metal
  • Method for detecting staphylococcus aureus enterotoxin by nanostructured composite metal

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Embodiment Construction

[0033] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments. But the following examples are only limited to explain the present invention, and the protection scope of the present invention should include the entire content of the claims, and those skilled in the art can realize the entire contents of the claims of the present invention through the following examples.

[0034] Next, the localized surface plasmon resonance imaging nanostructure with nanostructure characteristic size less than 70 nanometers is fabricated by the method of the present invention, and the structure is used to realize chemical surface treatment and detect and identify SEB solution with a concentration of 0.1 μg / ml.

[0035] (1) The glass substrate 1 with a size of 10mm×10mm was warmed at 70°C for 20min in newly prepared Piranha solution (3:1 sulfuric acid:30% hydrogen peroxide) before use to remove surface impurities, and then de...

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Abstract

The invention discloses a method that detects Staphylococcus aureus enterotoxin by a composite nano metal structure, which comprises the following steps: (1) a substrate is chosen and cleaned, and a polystyrene nano-sphere layer is self-assembled on the substrate; (2) the produced self-assembled polystyrene nano-sphere layer is taken as a die, and a silver film and a gold film are vapor-depositedon the self-assembled layer through a vapor deposition method; (3) the self-assembled polystyrene nano-sphere layer is removed through a Lift-off technique, and the nano metal array structure is obtained after array transformation; (4) the nano metal layer is activated by adopting a non-directional covalent connection method, and a biological molecular film layer that resists SE sheep monoclonal antibodies IgG is formed on the surface of the nano metal structure; (5) extinction spectrum of the nano metal structure is extracted by a spectrograph, and an extinction spectrum peak before the biological combination is obtained; (6) a solution to be detected is dropped on the substrate surface and blow-dried after the reaction, and step (5) is repeated; (7) the extinction spectrum peaks that are obtained before and after step (6) are contrasted, thus determining whether the solution to be detected contains SE. The method that detects the Staphylococcus aureus enterotoxin with the composite nano metal structure does not need the purifying and the marking of the solution to be detected, and can be applied into the quick detection of SE or other toxins in the same type.

Description

technical field [0001] The invention relates to a detection method of staphylococcus aureus enterotoxin, in particular to a method for identifying staphylococcus aureus enterotoxin by utilizing localized surface plasmon resonance of metal nanostructures. Background technique [0002] Staphylococcus aureus enterotoxin, with a molecular weight of 25200-28300, has strong heat resistance and drug resistance. Ordinary heating methods cannot remove the toxin in food, so it can only be detected and controlled in the food control link . The detection of Staphylococcus aureus enterotoxin mainly includes latex agglutination, enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR). The double-antibody sandwich ELISA method is the most commonly used method for detecting Staphylococcus aureus enterotoxin. There are three necessary reagents in this assay method: (1) solid-phase antigen or antibody; (2) enzyme-labeled antigen or antibody, It is called "enzyme conju...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/55G01N21/41
Inventor 杨欢李飞罗先刚
Owner INST OF OPTICS & ELECTRONICS - CHINESE ACAD OF SCI
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