Use of Chinese potato as protein medicine lysozyme intestinal-tract sorbefacient
A technology for intestinal absorption and protein drugs, which can be used in drug combinations, peptide/protein components, medical preparations containing active ingredients, etc., and can solve problems such as low absorption efficiency
Inactive Publication Date: 2009-02-11
上海沈李科工贸有限公司 +1
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The absorption experiment of isolated rat jejunum tissue shows that, as a protein macromolecular drug, the bioavailability of lysozyme is only 1.47±0.20%, and the in vivo absorption efficiency is even lower
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Embodiment 1
[0017] The promotion effect of yam on intestinal absorption of lysozyme:
[0018] 1. Preparation and dosage of lysozyme preparation
[0019] Egg white lysozyme (20000U / mg)
[0020] Preparation method: prepare 2 mg / ml or 10 mg / ml oral drug solution with physiological saline, and store at 4°C for later use.
[0021] Dosage: 0.5mg-5.0mg / mouse
[0022] 2. Preparation and dosage of yam accelerator
[0023] Commercially available Chinese herbal medicine yam, according to the usage and dosage of "Chinese Pharmacopoeia".
[0024] Preparation method: decoction by conventional method, soak 120g yam in 100ml of cold water for 30 minutes, boil quickly and then decoct with slow fire for 20 minutes, centrifuge at low speed and take the supernatant to obtain the aqueous extract of yam, store at 4°C for later use.
[0025] Dosage: 0.2ml-2.0ml / mouse
[0026] 3. Determination of blood concentration of lysozyme
[0027] Determination method: lysozyme micrococcal colorimetric method, the li...
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Abstract
The invention discloses the application of yam as an accelerant for intestinal absorption of protein drug lysozyme. According to a normal method, commercially available yam is decocted, soaked and boiled and then centrifugated at low speed to get supernatant liquid, namely, the part of aqueous phase extract of the yam is the yam accelerant which is used for lavage and medication of stomach together with lysozyme according to a certain proportion. The experiments show that the yam can be served as the accelerant of intestinal absorption of macromolecular protein drugs to greatly promote the intestinal absorption rate of lysozyme so as to increase the blood concentration of lysozyme. The results of the experiments also show that the yam accelerant generally has no influences on the blood-drug background of mouse lysozyme since the body condition of the mouse is normal, and can obviously and effectively improve the intestinal absorption of the protein drug lysozyme at the same time, thus being an effective intestinal absorption accelerant. The experiments also indicate that the yam accelerant has no obvious influences on the metabolism of endogenous protein lysozyme and exogenous protein lysozyme drugs.
Description
technical field [0001] The invention relates to the new application of traditional Chinese medicine yam as an intestinal absorption accelerator, in particular to the application of yam as an intestinal absorption accelerator for protein drug lysozyme. Background technique [0002] Lysozyme, with a molecular weight of about 14kDa and an isoelectric point of 10.7, was first discovered by British bacteriologist Fleming in 1922. It is an alkaline enzyme that can hydrolyze mucopolysaccharides in pathogenic bacteria. The enzyme is widely found in egg whites of birds and poultry, tears of mammals, saliva, plasma, urine, milk and other body fluids, and microorganisms also contain this enzyme. Among them, the content of egg white is the most abundant, which is a kind of basic protein and stable to heat. [0003] Functions of lysozyme: 1) As one of the non-specific immune factors of the body, it participates in various immune reactions of the body and supplements the non-specific imm...
Claims
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IPC IPC(8): A61K36/8945A61K38/48A61P37/02A61P43/00
Inventor 陈宇光李静何佳沈彦萍
Owner 上海沈李科工贸有限公司