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Supports for assaying analytes and methods of making and using thereof

A support and molecular technology, applied in chemical instruments and methods, analytical materials, biological material analysis, etc., can solve problems such as feasibility concerns of anhydride polymers

Inactive Publication Date: 2009-02-18
CORNING INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For these reasons, there are potential concerns about the viability of using anhydride polymers for LID

Method used

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  • Supports for assaying analytes and methods of making and using thereof
  • Supports for assaying analytes and methods of making and using thereof
  • Supports for assaying analytes and methods of making and using thereof

Examples

Experimental program
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preparation example Construction

[0065] d. Preparation of support

[0066] The amount of binding polymer attached to the substrate can vary depending on the choice of binding polymer, biomolecule, and analyte to be detected. In one instance, the binding polymer comprises at least a monolayer. In yet another instance, the thickness of the bonding polymer is about 10 to about 2,000 . In another case, the lower end point of the bonding polymer thickness is 10', 20', 40', 60', 80', 100', 150', 200', 300', 400' or 500', and the upper The endpoints are 750', 1,000', 1,250', 1,500', 1,750', or 2,000', any lower endpoint may form a thickness range with any upper endpoint.

[0067] The binding polymer can be attached to the substrate using techniques well known in the art. For example, the substrate may be dipped in a solution of binding polymer. In another instance, the bonding polymer can be sprayed, vapor deposited, screen printed, or robotically pin printed or embossed onto the substrate. This can be done...

Embodiment 1

[0104] Example 1. Preparation of ethylene maleic anhydride copolymer pre-terminated with ethanolamine

[0105] Figure 13 shows the FTIR spectrum of the (ethylene-maleic anhydride) alternating copolymer (EMA) partially hydrolyzed and vacuum dried at 140°C for 1 hour. The EMA spectrum of the partial hydrolysis clearly shows the presence of carboxylic acid groups (located at 1790 cm -1 shoulder), which is due to hydrolysis during storage. The EMA spectrum after vacuum drying showed that all carboxyl groups had been converted to anhydrides (shoulder not visible). This carboxylic acid-free EMA is particularly suitable as a starting material to ensure a well-controlled pre-capping reaction.

[0106] 0.2 g of dry EMA purchased from Sigma Aldrich (ref. 18,805-0) was dissolved in 14.80 g of anhydrous 1-methyl-2- pyrrolidone (NMP). Meanwhile, add 239 μl of pure ethanolamine to 50 g of anhydrous NMP to prepare ethanolamine solution. Then, 5 grams of ethanolamine / NMP solution (which ...

Embodiment 2

[0108] Example 2. Preparation of a gold sensor chip for SPR detection

[0109] The Biacore gold sensor chips were cleaned with ethanol and water, then dried in a gentle stream of nitrogen. The chips were soaked in 11-mercaptoundecylamine in 1 mM ethanol solution for 1 hour, washed with ethanol, washed with water, and finally dried in nitrogen flow.

[0110] A silicone rubber gasket (Flexperm) was pasted on each gold chip, and 100 μl of the pre-blocked EMA solution prepared in Example 1 was added to each sample well. After 10 min of coupling, the chips were rinsed with ethanol and dried under nitrogen flow. Remove the silicone gasket after drying. At this point, the coated gold chips are ready to immobilize biomolecules without any activation steps.

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Abstract

Described herein are supports for assaying an analyte and methods of making and using thereof.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to U.S. Application Serial No. 60 / 754,747, filed December 29, 2005, entitled "Supports for Detecting Analytes and Methods of Making and Using the Same," which is incorporated by reference incorporated in this application. Background technique [0003] Detection using label-independent detection (LID) platforms (e.g., surface plasmon resonance (SPR) or resonant waveguide grating sensors) is typically performed in a two-step approach: (i) making one of the binding partners (e.g., a protein) Immobilizing on the surface of the sensor; and (ii) binding a ligand (eg, drug, protein, oligonucleotide) to the immobilized protein. Traditionally, coupling of biomolecules to surfaces involves the activation of carboxylic acid groups on the surface to reactive N-hydroxysuccinimide (NHS) esters, which are then coupled to amino groups on the protein of interest. This method has been successfully used a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543C08F8/00C08F22/06B01J19/00
CPCB01J2219/00725B01J2219/00722B01J2219/00612B01J2219/0072B01J2219/00736B01J2219/0061B01J2219/00659B01J2219/00641B01J2219/0074B01J2219/0063B01J2219/00637B01J2219/00596G01N33/54353B01J2219/00605B01J2219/00626B01J2219/00527B01J2219/00731C08F8/00C08F8/32Y10T436/143333C08F210/02C08F222/06
Inventor A·G·弗鲁托斯D·享利
Owner CORNING INC
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