Extraction of nano pearl organic matrix and molecular-weight gradation method

Abstract

The invention discloses a method for extracting a nano-pearl organic substrate and grading by molecular weight. Nano-pearl powder is added with water for twice with stirring to ensure that the nono-pearl powder is fully extracted, a suspension is obtained through a centrifugation with high-speed rotation, impurities are filtered, and then a first pearl organic substrate extract with a molecular weight more than 5kDa and a second pearl organic substrate extract with a molecular weight less than 5kDa are separated through a centrifugal concentration separation by a centrifugal concentration filter-membrane device; further an ammonium sulfate precipitation method is used to separate pearl protein and protein which is failed to be precipitated, and Sephadex G25 gel is used for chromatography according to the molecular weights of the proteins to obtain three grades: grade A, grade B, and grade C. The method ensures that industries such as cosmetics, food and so on, can obtain pearl protein or pearl peptide with purified effective compositions for best utilization.

Description

technical field [0001] The invention relates to a method for easily obtaining pearl protein or pearl peptide with purified active ingredients in industries such as cosmetics and food, in particular to a method for extracting and molecular weight grading of nano-pearl organic matrix. Background technique [0002] Regardless of pearl or mother-of-pearl, the outer skin cells of the mantle are the only basic material for its formation. Commercially available micron-sized pearl powder or mother-of-pearl powder, 95% to 99% is composed of aragonite crystal calcium carbonate plus a small amount of calcite crystal calcium carbonate, and only contains 1% to 5% polysaccharide and protein organic matrix. Therefore, in the cosmetics, food and other industries, when it is desired to use the active ingredients of the pearl organic matrix as a preparation, the commercially available micron-sized pearl powder or mother-of-pearl powder is difficult to implement directly because the organic ma...

AI Technical Summary

Problems solved by technology

Therefore, in the cosmetics, food and other industries, when it is desired to use the active ingredients of the pearl organic matrix as a preparation, the commercially available micron-sized pearl powder or mother-of-pearl powder is difficult to implement directly because the organic matrix content is not high or unstable or has not been purified. use

Although the person in charge of the applicant company has successfully developed nano-scale pearl powder, such as the patent case of Taiwan Patent Invention Certificate No. 201397 "A Method for Making Edible Pearl Powder", it is still in the stage of direct consumption and easy digestion and absorption. In order to use the active ingredients of its pearls in the industry, it will still be difficult to implement under the conditions of economic benefits because the content of the organic matrix of the pearls is not clear and has not been purified.

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