Method for improving streptomyces bambergiensis bambermycin yield and bacterial strain

A technology of Streptomyces canola and flavomycin, which is applied to the field of increasing the yield of Streptomyces canola, can solve the problems of increasing the yield of Streptomyces canola, and achieves a convenient and quick genetic operation method and increases the output. , the effect of overcoming blindness

Active Publication Date: 2013-04-10
内蒙古中牧生物药业有限公司
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current literature, there is no report on using this gene to increase the production of flavomycin in Streptomyces spectrum

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for improving streptomyces bambergiensis bambermycin yield and bacterial strain
  • Method for improving streptomyces bambergiensis bambermycin yield and bacterial strain
  • Method for improving streptomyces bambergiensis bambermycin yield and bacterial strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0018] Example: Improving Flavomycin Production by Interrupting the nsdA Gene in a Streptomyces spectae strain

[0019] (1) PCR cloning of a fragment comprising the nsdA gene from the genomic DNA of Streptomyces spectrum

[0020] (a) inoculate Streptomyces spectrum ZM2006 in 30mLTSB culture medium (as described later), cultivate 36hr at 37 ℃, collect mycelium, extract genomic DNA; Template, the universal primer P obtained by bioinformatics analysis 1 : GAAGATCTCATGGACGAGCTGGGCAA and P 2 : GAAGATCTCTTGATCTCGTCGAGCCG PCR amplification, PCR reaction program: 95 ℃ pre-denaturation 5min; 95 ℃ denaturation 45 seconds, 58 ℃ renaturation 1min, 72 ℃ extension 5min, 30 cycles; 72 ℃ extension 5min. Agarose gel electrophoresis detection, a band of about 4.7kb was obtained. (c) carry out agarose gel recovery with the 4.7kb band that (b) obtains, carry out enzyme linkage reaction with carrier pMD18-T (the AT cloning kit purchased from TaKaRa Company); (d) obtain with (c) The enzyme-link...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of industrial microorganism molecular breeding, in particular to a method for improving the yield of plaque streptomyces flavomycin by means of molecular biology and the breeding of high-yield strains. The method comprises the following steps: establishing an effective conjugal transfer system between escherichia coli and plaque streptomyces; cloning the segmentcontaining nsdA homologous genes from the genome DNA of the plaque streptomyces by universal primers to obtain a recombinant vector containing the nsdA homologous gene segment; constructing the recombinant vector with damaged nsdA genes; transferring the vector into the plaque streptomyces; obtaining the plaque streptomyces strains with the damaged nsdA homologous genes through resistance screening; and performing flavomycin fermentation test to the plaque streptomyces strains to obtain the high-yield flavomycin strains. The plaque streptomyces is preserved in CGMCC. The method solves the defects in the prior art and provides a new approach for transforming the plaque streptomyces and improving the yield of the flavomycin by gene engineering.

Description

technical field [0001] The invention belongs to the field of molecular breeding of industrial microorganisms, and in particular relates to the optimization of Escherichia coli-Streptomyces spectrum conjugation transfer system, the breeding of high-yield Streptomyces spectacles strains, the construction of intermediate vectors and the improvement of the yellow sage Methods for Mycin Production. The present invention utilizes genetic engineering means to destroy a gene to reach the method for improving the flavomycin level produced by Streptomyces spectrum. Streptomyces avermitilis) is a negative regulator of antibiotic production. The invention is based on destroying the homologous gene of nsdA in Streptomyces spectacles, and improving the output of flavomycin by using the transformed bacterial strain. Background technique [0002] Streptomyces belong to a class of industrial microorganisms of great commercial importance. Nearly 70% of antibiotics in nature are produced by...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/09C12N15/31C12N1/21C12R1/465
Inventor 张国栋曹芹郑应华齐鹏闵勇
Owner 内蒙古中牧生物药业有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap