Kit for qualitatively detecting goldfish vitellogenin, preparation method and application thereof

Abstract

The present invention discloses a kit for the qualitative detection of vitellogenin and a preparation method and application thereof. When the kit is prepared, a pure product of the vitellogenin and the anti-mouse vitellogenin polyclonal antibody are prepared first, and then one pure product of the vitellogenin, one anti-mouse vitellogenin polyclonal antibody, one PVDF film, a color developing solution, a confining liquid, a washing solution, one feminine compare serum and one goat anti-rat labeled with horse radish peroxidase are put into a case body to obtain the kit for the qualitative detection of the vitellogenin. The kit for the qualitative detection of the vitellogenin can be applied to the investigation of endocrine disrupting chemicals. The kit can sensitively, accurately, conveniently and qualitatively detect the vitellogenin in the culture liquid of blood, liver tissue and liver cells of a goldfish. The lowest detection limit is 100 ng mL<-1>.

Description

technical field [0001] The invention relates to a kit for qualitatively detecting goldfish vitellogenin, a preparation method and application thereof. Background technique [0002] With the continuous improvement of the degree of industrialization and the living standards of the people in our country, a large amount of industrial wastewater and domestic sewage are discharged into rivers, lakes and seas, causing serious water environmental pollution, which not only endangers the survival of wild creatures, but also seriously threatens the life of our people. healthy. Although the concentration of these pollutants in the environment is very low, they can interfere with a series of biological processes such as the synthesis, release, transportation, and metabolism of hormones in humans and animals, as well as the combination of hormones and receptors, and the expression of functions, thereby disrupting human and animal health. The functions of animal endocrine system, nervous ...

AI Technical Summary

Problems solved by technology

[0004] At present, several kinds of ELISA kits and radioimmunoassay kits for vitellogenin of teleosts have been established at home and abroad, but these kits have higher requirements for equipment; and because fish vitellogenin has a strong Due to the differences in species specificity and geographical distribution of fish, it is necessary to prepare and use a detection kit that is easy to use, does not require high equipment, and is aimed at the detection of vitellogenin in local fish that is widely distributed in my country.