Putuo hornbeam gemma culture medium and tissue culture rapid propagation method

A technology of Putuo hornbeam and culture medium, which can be applied to plant cells and other directions, and can solve the problems of slow speed and insufficiency.

Inactive Publication Date: 2009-04-08
ZHEJIANG FORESTRY UNIVERSITY
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Problems solved by technology

At present, after many years of seedling and afforestation experiments, 379 seedlings have been cultivated by sowing, cutting, grafting, etc., but the speed is too slow to meet the urgent needs of rapid recovery of this rare species. Exploring and trying to expand the tree by tissue culture and rapid pro

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  • Putuo hornbeam gemma culture medium and tissue culture rapid propagation method

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Embodiment Construction

[0015] The present invention will be further described in conjunction with the following examples: MS salts and MT vitamins are commercially available and used according to the conventional method shown in the product description, which is commonly used in the industry. But in the MS used in the present invention, only use its 1 / 3 weight macroelement salt and the trace element salt of whole amount, for the sake of brevity, represent with 1 / 3MS salt. The present invention does not contain IBA in the bud survival growth medium that uses respectively in different cultivation stages, does not contain 1 / 3MS salt in the rooting medium of the first stage, does not contain IBA in the rooting medium of the second stage, this aspect is because three The first stage is carried out continuously. The 1 / 3MS salt or IBA used in the previous stage still has an effect on the tissue culture, and it can be used in the latter stage. On the other hand, after repeated comparative tests, it is more ...

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Abstract

The invention relates to a culture medium for carpinus putoensis gemmas which is prepared by a 1/3MS salt, an MT vitamin, an IBA (indolebutyric acid) which is 0mg/l to 60mg/l, an inose which is 50mg/l to 150mf/l, a gelrite which occupies 0.2 percent to 0.4 percent of the total weight of the culture medium and a cane sugar which occupies 1.5 percent to 4.5 percent of the total weight of the culture medium. The culture medium can culture abacterial test-tube gemmas effectively and induce rooting, thereby rapidly culturing and reproducing a mass of regrowth. The process for tissue-culturing and rapidly reproducing carpunus putoensis by using the tissue culture medium comprises the following four steps: the first one is the collection and disinfection of explants, the second one is the culture of abacterial gemmas, the third one is the root induction with binary form and the last one is the domestication of transplants. The invention firstly takes the gemmas as explants to tissue-culture and rapidly reproduce carpinus putoensis saplings, which overcomes the physical disabilities of the tree like bad germination, short life of pollen, low sprout rate and insufficient effective pollination period and provides biological engineering technical measures for the reproduction and preservation of the tree, thereby saving the carpinus putoensis species resource which is almost extinct.

Description

【Technical field】 [0001] The invention relates to a tissue culture medium and a tissue culture rapid propagation method, which is aimed at the bud body culture medium and the tissue culture rapid propagation method of the endangered arbor, Putuo hornbeam. 【Background technique】 [0002] Putuo hornbeam (Carpinus putoensis) belongs to the genus CarpinusL and belongs to Beturaceae. Putuo hornbeam is a rare tree species unique to my country. Its distribution range is extremely narrow. It is only found in Zhoushan Islands, Zhejiang. It is one of the 10 nearly extinct tree species in my country. Due to the poor germination of the tree, short pollen life, pollen vigor declines within seven days, and the seed germination rate is low, it is endangered by factors such as ecological and human damage. According to survey data, there is only one wild Putuo hornbeam left in Mount Putuo. In view of this, the species protection of Putuo hornbeam has attracted the attention of botanical re...

Claims

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Application Information

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IPC IPC(8): C12N5/04
Inventor 黄丽春俞慈英张翠萍李修鹏林新春
Owner ZHEJIANG FORESTRY UNIVERSITY
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