Process for producing fire resistant alpha-diastase

Abstract

The invention discloses a preparation method of thermostable Alpha-amylase, pertaining to the technical field of bioengineering enzyme preparation. The preparation method uses bacillus licheniformis as strains and corns as carbon and nitrogen sources to produce the Alpha-amylase of high temperature resistance and high activity by fermenting in a first-grade seed tank, batch fermenting with feeding in big tank, fine filtering and sterilizing process. The fermentation capacity can reach a top of 13480u / ml, with an average level of enzymatic production with fermentation of 12360u / ml. The capacity is 54.5 percent higher than the fermenting level which is 7982u / ml before the prior art is optimized. Every standard ton of enzyme consumes 19.8kg of starch, being 5kg less than the batch fermenting which consumes 24.8kg, thus forming a complete set of fermenting feeding batch technique of thermostable Alpha-amylase and realizing the industrial scale production of thermostable Alpha-amylase.

Description

technical field [0001] The invention relates to a production method of a bioengineering enzyme preparation, in particular to a production method of a high-temperature-resistant alpha-amylase. Background technique [0002] As a new type of enzyme preparation, high temperature resistant α-amylase has wide application and good market prospects in fermentation and grain deep processing industries. It is currently widely used in alcohol, beer, monosodium glutamate, organic acid, starch sugar and pharmaceutical industries. And sales are increasing year by year. Since the 1980s, my country has started research on the production technology of high-temperature-resistant α-amylase. The Shanghai Institute of Industrial Microbiology has used Bacillus licheniformis as the starting strain to increase the enzyme production unit from 1-2 units / ml to 100 units / ml. about milliliters. It was not until 1989 that foreign strains were introduced for industrial production. After more than ten yea...

AI Technical Summary

Problems solved by technology

It was not until 1989 that foreign strains were introduced for industrial production. After more than ten years of hard work, the fermentation activity has increased from less than 500u / ml to 8000u / ml, and some manufacturers have reached 8500-9000u / ml, but this is different from the United States, Denmark and other countries. Compared with the high temperature resistant α-amylase fermentation level of 25000u / ml, there is still a big gap

Lin Jian, Cui Jingchun, etc. (Journal of Wuxi University of Light Industry, July 2003, Volume 22) improved the fermentation activity by adjusting the culture medium formula or fermentation process, but did not optimize the medium and fermentation process at the same time (Food and Fermentation Industry, No. 33, Phase 7, Volume 33, 2007) optimized the fermentation medium and process by shaking flask experiments, and verified it in a 50L fully automatic fermenter , the fermentation activity increased by 14%, reaching 14900u / ml, without carrying out larger pilot and industrial trial production

And Chinese patent 2007100361599 has reported a kind of high-activity, high-purity high-temperature-resistant α-amylase production process, adopts secondary fermentation, base material is fed feed, pulse feeds ammonia, uses the technology of cooked soybean meal as nitrogen source, adopts the technology of controlling fermentation temperature Fermentation at 43°C, pH 6.8, high temperature resistant α-amylase product is obtained through flocculation, pressure filtration, circulating primary filtration, and fine filtration. The process is relatively complicated, which affects its large-scale promotion

[0004] At present, in the process of industrialized production of high-temperature-resistant α-amylase, due to the lack of systematic theoretical basis, manufacturers mostly guide production based on production experience, production management and technical guidance are blind, and high-temperature-resistant α-amylase belongs to the middle Non-toxic fermentation environment, the success rate of fermentation is not high, resulting in high production costs and weak market competitiveness

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