Instrument and reagent for fast detection of microbe in blood specimen, and preparation method thereof

Abstract

The invention discloses an instrument and a reagent used for quickly detecting microorganisms in blood samples on the basis of a piezoelectric sensor principle. The testing instrument is characterized in that the sensitiveness is high, digitalization is easy to be realized, response spectrum is wide, the detection is quick, the operation is simple and the cost is low and the like; the testing instrument sufficiently utilizes the response of a serial connection typed piezoelectric sensor on electric conduction of solution and combines with the metabolism characteristics of the microorganisms, broadens the application of the piezoelectric sensor on critical blood detection and provides reliable bases for the diagnosis and application of the disease; and the reagent matched with the instrument is characterized in that the reagent is formed by the configuration of beef extract, yeast extract, YC-1 solution and YC-2 solution; the proportion of all components is 0.2 to 10g / L of beef extract, 0.2 to 10g / L of yeast extract, 20 to 60g / L of YC-1 solution and 10 to 40g / L of YC-2 solution. The invention also discloses a preparation method used for the reagent.

Description

technical field [0001] The invention belongs to the fields of chemical reagents, detection and computer applications, and relates to an instrument, reagent and preparation method for rapid detection of microorganisms in blood samples. Background technique [0002] The abuse of clinical antibiotics in our country has been quite serious. According to the survey results of the Chinese Hospital Infection Management Committee, the utilization rate of antibiotics in my country has reached 70% in tertiary hospitals, 80% in secondary hospitals, and 90% in first-class hospitals. At the same time, the statistical report of the World Health Organization is 30%, and the statistical result of the United States is only 20%. The irrational use of clinical antibiotics not only increases the economic burden of patients, but also causes a huge waste of medical resources; more seriously, it leads to Many clinical adverse reactions and the continuous growth of drug-resistant bacteria. [0003...

AI Technical Summary

Problems solved by technology

For weak bacteria such as fastidious bacteria and fungi, it is easy to cause false negatives [6]; for some non-bacterial factors that cause the increase of CO2 concentration in the culture bottle (such as white blood cell overgrowth in the blood of leukemia patients, severe Elevation of whole blood leukocytes caused by infection, unstable culture temperature, etc.) often cause false positives[7]

In addition, the instruments and reagents of the fully automatic microbial culture system are very expensive, which seriously restrict the promotion and application of the fully automatic culture system

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