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Pharmaceutical composition for preventing and treating atherosclerosis

A technology of atherosclerosis and composition, applied in the direction of drug combination, active ingredient of heterocyclic compound, pharmaceutical formulation, etc.

Active Publication Date: 2009-04-22
北京赛德维康医药研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Recent studies have found that, in addition to the classic risk factors such as hypertension and hyperlipidemia, hyperglycemia and hyperhomocysteine ​​are high-risk factors for atherosclerosis, so the above-mentioned treatment measures have their therapeutic value. limitations

Method used

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  • Pharmaceutical composition for preventing and treating atherosclerosis
  • Pharmaceutical composition for preventing and treating atherosclerosis
  • Pharmaceutical composition for preventing and treating atherosclerosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Isolation and culture of bovine aortic endothelial cells: newborn calves were sacrificed by bloodletting from the carotid artery, and the calf aorta was taken out under aseptic operation, and placed in normal saline containing penicillin and streptomycin at 4°C to remove adhesions and adipose tissue. Rinse with normal saline, cut off a 10cm-long blood vessel segment, ligate the branch and the opening of the lower segment, inject 0.1% type I collagenase solution, put it in a constant temperature water bath at 37°C for 15 minutes, collect the digestive juice, and rinse it with D-Hanks, the liquid is mixed with Combine the digestion solutions, centrifuge at 1200r / min for 10min, discard the supernatant, suspend the cells in DMEM medium containing 10% new serum, plant them in a culture bottle, and place them at 37°C, 5% CO 2 Cultured in an incubator, the cells grew to confluence, passaged at a ratio of 1:2, and the endothelial cells that grew well for 3-5 passages were collec...

Embodiment 2

[0034] According to the screening research results of Chinese medicine prescriptions, the combination of arecoline, ligustrazine and paeonol in the proportioning ratio within the effective range in the above-mentioned research is selected, arecoline in terms of molar mass: ligustrazine: paeonol=1:30 :40 (referred to as EPD), it is mixed with aqueous solution (the configuration method of the prescription is all the same in the following biologically active embodiments), and further observes the bovine aortic endothelial cell caused by HCY by the EPD three-drug prescription The effect of pathological overexpression of MCP-1, ICAM-1, VCAM-1.

[0035] The isolation and culture of bovine aortic endothelial cells were the same as in Example 1. The 3rd generation bovine aortic endothelial cells in good growth state were selected for the experiment, and the cells were divided into 2×10 5 cells / ml seeded in 6-well plate, 1.5ml per well, the total number of cells is 3×10 5 After the c...

Embodiment 3

[0038] The test uses big-eared white rabbits, ♂, weighing 1.5-2.0kg. Before the experiment, there was no statistical difference in blood lipid levels of animals in each group. During the 4th week of the experiment, the serum total cholesterol level of the rabbits fed with a high-fat diet was 11.76 times that of the normal diet group. During the 2-3 months of the experiment, a high-level steady state was reached, indicating that the hyperlipidemia of the rabbits had completely disappeared during the 4th week of the experiment. form. Under the same experimental conditions, oral administration of simvastatin 5mg / kg can significantly reduce the serum total cholesterol level in rabbits, and oral administration of EPD 0.2mg / kg, 0.4mg / kg, 0.8mg / kg has no significant effect on the rabbit serum total cholesterol level ( image 3 ).

[0039] At the end of the 12th day, the rabbits were killed by air embolism, and the aorta, from the aortic arch to the bifurcation of the common iliac ...

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Abstract

The invention relates to a medicine composition containing arecoline, ligustrazine and paeonol, and application thereof to preparation of medicine resisting atherosclerosis. The medicine composition is suitable for preventing and treating the atherosclerosis and cardiovascular diseases related to the atherosclerosis.

Description

technical field [0001] The invention relates to a pharmaceutical composition for preventing and treating atherosclerosis, a preparation method thereof, and an application of the composition in treating diseases related to atherosclerosis. Background technique [0002] Atherosclerosis is the common pathophysiological basis of stroke, coronary heart disease, and peripheral vascular disease. 50% of the causes of death in European and American countries are closely related to it, and its incidence is on the rise in my country. The resulting death and disability caused by acute cardiovascular and cerebrovascular events have brought huge social and economic burdens to the country and individuals . According to the calculation of the health department, the direct medical expenses for stroke in my country can reach 25 billion yuan each year. [0003] For more than half a century, the research on the prevention and treatment of atherosclerosis has always been the research focus of t...

Claims

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Application Information

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IPC IPC(8): A61K31/4965A61P9/10A61P19/06A61K31/12A61K31/44
Inventor 汪海王倩段智变石翠格
Owner 北京赛德维康医药研究院
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