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Bionic dermis as well as preparation method and application thereof

A dermal and bionic technology, applied in the field of medical and surgical repair materials, can solve the problems of lack of mechanical properties of skin and dermis

Inactive Publication Date: 2009-04-22
RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are still some skin substitutes made of Chinese herbal medicines, but they lack the mechanical properties of normal skin dermis

Method used

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  • Bionic dermis as well as preparation method and application thereof
  • Bionic dermis as well as preparation method and application thereof
  • Bionic dermis as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Preparation of Bionic Dermis I

[0078] Normal tissue skin was decellularized and then homogenized. This normal tissue contains various cell growth factors mentioned above, proteoglycans, glycoproteins, and protein molecules that promote cell and fiber adhesion.

[0079] Three-dimensional multi-directional (4, 5, 6, 7) weaving of different fibers, and then immersed in the homogenate prepared above, at room temperature, after 30 minutes, taken out and dried. Get the dermis template as described. The area can be determined according to the area of ​​the wound.

[0080] Then the dermal template was implanted into the wound, the superficial blade-thick skin was grafted, and bandaged under pressure.

Embodiment 2

[0082] Preparation of Bionic Dermis II

[0083] Dip different fibers 3, 4, 5, 6, 7, 8, 9, 10, and 11 into fibronectin (20-200 micrograms / ml), collagen (10-100 micrograms / ml), in vitro fibronectin (vitronectin, VN) (1-50 micrograms / ml), hyaluronic acid (2-50 micrograms / microliter), non-collagenous glycoproteins, elastin, syndecan, aminoglycans, glycoproteins and growth factors, and Immerse in PEG or PLL-g-PEG (1mg / ml), then take it out, dry it, make different combinations, three, or five or seven, each combination contains PEG or PLL-g-PEG, and then edit The braids are made into fiber bundles, and then the fiber bundles are three-dimensionally multi-directional, five-directional, and seven-directional to weave to make a leather template (that is, bionic leather II). The area can be determined according to the area of ​​the wound.

[0084] Then the dermal template was implanted into the wound, the surface was grafted with autologous slash-thick skin, and bandaged under pressur...

Embodiment 3

[0088] Animal experiments of bionic leather

[0089] In this embodiment, animal experiments are used to test, and the test method is as follows: select a certain number of clean Duroc pigs (purchased from: Shanghai Slack Experimental Animal Co., Ltd.), 24 hours after the back hair removal, use a scalpel on the back of the pig A full-thickness skin of a certain size was cut, and the blade-thick skin was taken back on a drum-type dermatome as autologous skin. The wounds were randomly divided into four groups:

[0090] The wound in group 1 is the experimental wound. After hemostasis, the bionic dermis prepared in Example 1 was first transplanted, and then the autologous skin was transplanted on it, and bandaged under pressure;

[0091] The wound in group 2 is the experimental wound. After hemostasis, the bionic dermis prepared in Example 2 was first transplanted, and then the autologous skin was transplanted on it, and bandaged under pressure;

[0092] The wound in group 3 was ...

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Abstract

The invention discloses bionic dermis. An implant is a three-dimensional braided fabric of biocompatible fiber, and the fiber adsorbs, absorbs or is wrapped with protein substance favorable for skin or dermis restoration. The invention also discloses a preparation method for the bionic dermis and application thereof.

Description

technical field [0001] The invention relates to a medical surgical repair material, in particular to a bionic corium and its preparation method and application. Background technique [0002] The skin covers the surface of the whole body and is in direct contact with the external environment. It is an important boundary organ in anatomy and physiology. The skin is composed of epidermis and dermis, connected with deep fascia, aponeurosis or periosteum by subcutaneous tissue. The skin originates from the ectoderm and mesoderm, and has a complex and highly specialized structure. It has an important barrier and protective function, which can prevent external stimuli from damaging tissues in the body, block the invasion of foreign bodies and microorganisms, and prevent the extravasation of body fluids and Absorption of foreign substances. [0003] As the largest organ of the human body, the skin is a barrier to contact with the outside world. When skin defects are caused by fact...

Claims

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Application Information

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IPC IPC(8): A61L27/60A61L27/54A61L27/14
Inventor 姜育智陆树良
Owner RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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