Method for rapidly detecting biochemical oxygen demand by using on site culture organism

Abstract

The invention provides a method used for detecting the oxygen demand for quick biochemistry by cultivating microorganism in situ. The in-situ microorganism culture is defined as that the pipeline carrying current in a flowing system is taken as a carrier, the flowing of water sample containing organic matters and microorganism is taken as precondition, the microorganism in the water sample is adsorbed onto the surface of the internal wall of the pipeline. The pipeline carrying current in the flowing analysis system is taken as the carrier to adsorb on the microorganism on the spot in the environment water; on the one hand, the adsorbed microorganism has idiosyncratic degradation capability on the organic matter in the water and the obtained result has higher pertinence; on the other hand, the adsorption state of the microorganism on the internal wall of the pipeline is most approaching to the natural surviving state and the activity of the microorganism is kept to the most extent. The service life of the existing biological film of a BOD sensor is not more than 3 months and the maintenance is troublesome. in the method, the culture of microorganism in laboratory is saved, and a plurality of problems such as preparation, storage, activation and the like of the microorganism film; furthermore, the adsorbed microorganism can always keep the physiological activity state during the continuous analysis and testing process, therefore, the method has extremely wide application prospect.

Description

technical field [0001] The invention relates to a method for on-site cultivation of microorganisms to detect biochemical oxygen demand (BOD). It is a method based on the flow analysis system to quickly detect BOD by absorbing aerobic microorganisms in water samples with a flow-through pipeline as the substrate. Background technique [0002] Biochemical oxygen demand is an important indicator for analyzing the content of organic pollutants in water bodies, and is one of the most important parameters in routine monitoring of water quality. The current standard dilution method (BOD 5 method) has many deficiencies, such as long time, heavy workload, cumbersome operation, many interference factors, poor repeatability, and cannot reflect water quality changes in time, and cannot realize online monitoring of water quality. [0003] Since Japanese scientist Isao Karube developed the first BOD microbial sensor in 1977, many research groups at home and abroad have developed BOD micr...

AI Technical Summary

Problems solved by technology

This method limits the application of BOD biosensors due to the type and quantity of microorganisms and the properties of immobilized materials.

For example, biofilm requires specific preparation technology, low-temperature storage, preservation and short service life, and long activation before use, which brings difficulties in application.

On the other hand, the composition and content of organic pollutants in environmental water vary greatly, and the microorganisms immobilized by the sensor have significantly different respiration rates for different organic substances, thus limiting the scope of application of BOD biosensors.

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