Method for rapidly detecting biochemical oxygen demand by using on site culture organism

A technology of biochemical oxygen demand and on-site cultivation, which is applied in the field of on-site cultivation of microorganisms to detect biochemical oxygen demand, can solve the problems of limitation, preservation, short service life, and difficulty in activation, so as to prolong the service life, improve practicability, and practicality Apply promising effects

Active Publication Date: 2009-04-22
吉林市光大分析技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method limits the application of BOD biosensors due to the type and quantity of microorganisms and the properties of immobilized materials.
For example, biofilm requires specific preparation technology, low-temperature storage, preservation and short service life, and long activation before use, which brings dif

Method used

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  • Method for rapidly detecting biochemical oxygen demand by using on site culture organism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Detection of milk factory water samples

[0027] The sample cup 14 is pre-filled with 300ml milk factory water sample, and it is air-saturated at a rate of 3L / min. The current-carrying tube 8 is a plastic tube with a length of 180 cm and a material of ethylene-vinyl acetate copolymer (EVA), with an inner diameter of 1.2 mm. The current-carrying tube 8 is placed in a constant temperature water bath with a temperature of 30°C. After the cultivation started, the peristaltic pump 9 delivered the water sample from the water sample cup 14 to the surface of the oxygen electrode 5 through the current-carrying tube 8 at a flow rate of 1.0ml / min. The flow rate of min is supplied to the water sample cup 14. After culturing for 48h, the BOD 5The 20.0mg / L GGA standard solution was injected into the standard solution cup 16, the 5mM phosphate buffer solution was injected into the buffer solution cup 15, and both were saturated with air at 3L / min. Change the flow directi...

Embodiment 2

[0029] Example 2 Detection of settled biochemical aeration tank water samples in urban sewage treatment plants

[0030] The water sample cup 14 is pre-filled with 300ml of settled biochemical aeration tank water samples from urban sewage treatment plants, and is saturated with air at a rate of 3L / min. The current-carrying tube 8 is a glass tube with a length of 120 cm and an inner diameter of 1.6 mm. First, the inner wall of the carrier tube 8 is covered with HF / NH 4 The F solution (1.7%:2.3%, w:w) corrodes and roughens it. The current-carrying tube 8 is placed in a constant temperature water bath with a temperature of 37°C. After the cultivation started, the peristaltic pump 9 delivered the water sample from the water sample cup 14 to the surface of the oxygen electrode 5 through the current-carrying tube 8 at a flow rate of 0.5ml / min. The flow rate of min is supplied to the water sample cup 14. 24 hours after the on-site culture was established, the BOD 5 The 5.0mg / L GG...

Embodiment 3

[0032] Embodiment 3 detects the water sample of the sewage treatment plant through primary sedimentation and coarse filtration

[0033] The water sample cup 14 is pre-filled with 300ml of water samples from the sewage treatment plant that have undergone primary sedimentation and coarse filtration, and are saturated with air at a rate of 3L / min. The current-carrying tube 8 is a glass tube with a length of 120 cm and an inner diameter of 1.6 mm. First, the inner wall of the carrier tube 8 is covered with HF / NH 4 The F solution (1.7%:2.3%, w:w) corrodes and roughens it. Inject 1M NaOH into the cleaned glass tube and continuously sonicate for 30 min, then fully wash with deionized water. 2 mg of carboxylated multi-walled carbon nanotubes (MWNTs) were dissolved in 10 ml of 1.0 mg / mL polydiallyldimethylammonium chloride (PDDA) and 0.5 M NaCl aqueous solution and sonicated for 3 hours. After centrifugation at 15000rpm for 10min, the MWNTs-PDDA complex was collected and fully washe...

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Abstract

The invention provides a method used for detecting the oxygen demand for quick biochemistry by cultivating microorganism in situ. The in-situ microorganism culture is defined as that the pipeline carrying current in a flowing system is taken as a carrier, the flowing of water sample containing organic matters and microorganism is taken as precondition, the microorganism in the water sample is adsorbed onto the surface of the internal wall of the pipeline. The pipeline carrying current in the flowing analysis system is taken as the carrier to adsorb on the microorganism on the spot in the environment water; on the one hand, the adsorbed microorganism has idiosyncratic degradation capability on the organic matter in the water and the obtained result has higher pertinence; on the other hand, the adsorption state of the microorganism on the internal wall of the pipeline is most approaching to the natural surviving state and the activity of the microorganism is kept to the most extent. The service life of the existing biological film of a BOD sensor is not more than 3 months and the maintenance is troublesome. in the method, the culture of microorganism in laboratory is saved, and a plurality of problems such as preparation, storage, activation and the like of the microorganism film; furthermore, the adsorbed microorganism can always keep the physiological activity state during the continuous analysis and testing process, therefore, the method has extremely wide application prospect.

Description

technical field [0001] The invention relates to a method for on-site cultivation of microorganisms to detect biochemical oxygen demand (BOD). It is a method based on the flow analysis system to quickly detect BOD by absorbing aerobic microorganisms in water samples with a flow-through pipeline as the substrate. Background technique [0002] Biochemical oxygen demand is an important indicator for analyzing the content of organic pollutants in water bodies, and is one of the most important parameters in routine monitoring of water quality. The current standard dilution method (BOD 5 method) has many deficiencies, such as long time, heavy workload, cumbersome operation, many interference factors, poor repeatability, and cannot reflect water quality changes in time, and cannot realize online monitoring of water quality. [0003] Since Japanese scientist Isao Karube developed the first BOD microbial sensor in 1977, many research groups at home and abroad have developed BOD micr...

Claims

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Application Information

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IPC IPC(8): G01N27/409G01N1/28
Inventor 刘长宇董绍俊
Owner 吉林市光大分析技术有限责任公司
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