Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for fluorescence labeling hydroxylapatite

A technology of hydroxyapatite and fluorescent labeling, which is applied in fluorescence/phosphorescence, diagnostic recording/measurement, preparation of test samples, etc. It can solve the problems of complex preparation methods and achieve good biocompatibility

Inactive Publication Date: 2010-09-08
ZHEJIANG SCI-TECH UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Publication number: CN1693410A", or terbium, europium, etc. "DoatA., Fanjul M., PellieF., Hollande E., Lebugle A., Biomaterials 2003, 24, 3365; Riwotzki K., Meyssamy H., Schnablegger H.Angew Chem lnt Ed.2001, 40, 573."Rare earth ions are doped into hydroxyapatite to make it emit fluorescence, etc., but the preparation methods of these materials are usually complicated, and the biocompatibility of quantum dots and rare earth elements yet to be verified

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for fluorescence labeling hydroxylapatite
  • Method for fluorescence labeling hydroxylapatite
  • Method for fluorescence labeling hydroxylapatite

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for fluorescently labeling hydroxyapatite, using spherical hydroxyapatite particles with a diameter of about 40 nanometers, and the labeling process is as follows:

[0019] (1) Dissolve 1.5 grams of analytically pure sodium bicarbonate in 50 milliliters of deionized water to obtain a buffer solution, which is stirred with a magnetic stirrer until completely dissolved;

[0020] (2) Take 1 mg of fluorescein isothiocyanate powder, dissolve it in the sodium bicarbonate solution in the first step above, and stir magnetically for 30 minutes in a dark environment.

[0021] (3) Take 75 mg of hydroxyapatite particles, put them into the fluorescein isothiocyanate solution prepared in the second step above, and stir magnetically for 0.5 hours in a dark environment;

[0022] (4) Place the suspension in step (3) above in a dark environment at 4°C for low temperature reaction for 10 hours;

[0023] (5) Centrifuge the suspension obtained in the above step (4) at 8000g to sep...

Embodiment 2

[0031] A method for fluorescently labeling hydroxyapatite, using irregular hydroxyapatite particles with a size of 200 nanometers, the labeling process is as follows:

[0032](1) 1.2 grams of analytically pure sodium bicarbonate are dissolved in 50 milliliters of deionized water to obtain a buffer solution, which is stirred with a magnetic stirrer until completely dissolved;

[0033] (2) Take 3 mg of fluorescein isothiocyanate powder, dissolve it in the sodium bicarbonate solution in the first step above, and stir magnetically for 30 minutes in a dark environment.

[0034] (3) Take 30 mg of hydroxyapatite particles, put them into the fluorescein isothiocyanate solution prepared in the second step above, and stir magnetically for 1.5 hours in a dark environment;

[0035] (4) React the suspension in step (3) above at 1°C in a light-proof environment for 20 hours;

[0036] (5) Centrifuge the suspension obtained in the above step (4) at 8000g to separate the labeled hydroxyapatit...

Embodiment 3

[0044] A method for fluorescently labeling hydroxyapatite, using needle-shaped hydroxyapatite particles with a size of 100 nanometers, the labeling process is as follows:

[0045] (1) 1.0 g of analytically pure sodium bicarbonate is dissolved in 50 milliliters of deionized water to obtain a buffer solution, which is stirred with a magnetic stirrer until completely dissolved;

[0046] (2) Take 2 mg of fluorescein isothiocyanate powder, dissolve in the sodium bicarbonate solution in the first step above, and stir magnetically for 30 minutes in a dark environment.

[0047] (3) Take 60 mg of hydroxyapatite particles, put them into the fluorescein isothiocyanate solution prepared in the second step above, and stir magnetically for 1.5 hours in a dark environment;

[0048] (4) React the suspension in step (3) above at low temperature for 20 hours in a light-proof environment at 8°C;

[0049] (5) Centrifuge the suspension obtained in the above step (4) at 8000g to separate the label...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Maximum absorption wavelengthaaaaaaaaaa
Maximum emission wavelengthaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for carrying out fluorescence labeling on hydroxyapatite. The method comprises the following steps: isothiocyanate fluorescein as a biological fluorescein is connected to a hydroxyapatite particle through adsorption, a covalent bond and other methods in order that the hydroxyapatite particle is labeled with fluorescence. The isothiocyanate fluorescein as a labeling substance adopted by the method is a biological fluorescein and has better biocompatibility; after long-time reaction and full washing, the isothiocyanate fluorescein and the hydroxyapatite particlecan realize firm combination; in the using process, the isothiocyanate fluorescein can not be separated from the surface of the hydroxyapatite so as to cause negative influence on a testing result; and the absorption peak of an obtained fluorescent substance has narrow range which is between 480 and 510 nanometers and is suitable for an environment of the coexistence of various fluorescence. The method can be applied in the field of material and biomedicine research and is in particular suitable for the research of an interface, a process and a mechanism when the hydroxyapatite particle and acell interact.

Description

technical field [0001] The invention relates to a method for fluorescently labeling hydroxyapatite. Background technique [0002] In nature, the main biomineral phase of vertebrate hard tissues is hydroxyapatite. Studies have found that synthetic hydroxyapatite has the same composition, structure and excellent biocompatibility as mineral phase hydroxyapatite, so it is considered to be one of the most ideal substitute materials for bone tissue repair, and has been used clinically. It is widely used in the repair and replacement of bone tissue loss and defect. [0003] In recent years, with the rapid development of analysis and detection methods and technologies, it has been found that the basic constituent units of hydroxyapatite particles that make up human hard tissues are maintained at the nanoscale—ordered hydroxyapatite particles embedded in collagen, Endows bone tissue with excellent biological and mechanical properties "Gao H., Ji B., Jager I.L., Arzt E., Fratz P., P...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N1/28G01N21/64A61B5/00
Inventor 蔡玉荣姚菊明李恭楚王秀华
Owner ZHEJIANG SCI-TECH UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com