Calcium transformation ratio increasing method in sheep bone using enzymolysis and fermentation

A conversion rate and enzymatic hydrolysis technology, applied in fermentation, application, fungi, etc., can solve the problems of low solubility and low ratio of effective utilization of sheep bone bone calcium, and achieve the effect of improving the conversion rate

Inactive Publication Date: 2009-05-06
TIANJIN AGRICULTURE COLLEGE
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AI Technical Summary

Problems solved by technology

Although sheep bone is rich in calcium, it is mainly composed of hydroxyapatite [Ca 10 (PO 4 ) 6 (OH) 2 ] It exists in the form of crystals with extremely low solubility, and due to synergistic problems with other nutrients, the proportion of sheep bone bone calcium that can be effectively utilized by the human body is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Fresh lamb bones: from Tianjin Zhongao Food Group Company.

[0026] Pepsin (activity unit 12,500 U / g) was purchased from Nuoao Enzyme Biological Co., Ltd.

[0027] Lactobacillus plantarum and Pediococcus pentosacea were from the Microbiology Laboratory of China Agricultural University.

[0028] Take 7.8kg of fresh sheep bones after drying and grinding, and make a 0.2kg / L solution with water, add 1185.6 grams of pepsin in the solution, adjust the enzymolysis temperature to 37°C, enzymolysis time to 6h, and pH value to 3.0. Enzyme hydrolysis, after enzymatic hydrolysis, inactivate the enzyme at 90°C for 10 minutes, cool to room temperature, and set aside.

[0029] Insert Lactobacillus plantarum and Pediococcus pentosaceae into liquid MRS medium sterilized at 121°C for 15 minutes at 1% inoculum, culture at 37°C, and the number of bacteria reached 10 after three generations of activation. 7 ~10 8 cfu mL -1 .

[0030] Take 20ml of liquid MRS to make No. 1 medium; take 4...

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Abstract

The invention relates to a method for improving the conversion rate of calcium in sheep bone by jointly utilizing enzymolysis and fermentation, comprising the following steps: 1) enzymolysis liquid of the sheep bone is prepared; 2) strains are activated; 3) the strains are acclimatized; and 4) the enzymolysis liquid of the sheep bone is fermented. The method can greatly increase the conversion rate of calcium in sheep bone, thus improving the effective utilization of calcium in sheep bone by human body. The product prepared by the method is a superior and effective raw material for enriching calcium. The raw material can be processed into foods, drinks or health care preparations through various conventional methods.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for improving the conversion rate of calcium in sheep bones by combining enzymolysis and fermentation technologies. Background technique [0002] my country is a large animal husbandry country, and the resources of livestock and poultry bones are extremely rich. However, when people consume livestock and poultry meat, a large number of livestock and poultry bones are not utilized. This not only wastes rich nutrients such as bone protein and bone calcium, but also wastes The animal bones have polluted the environment again. Therefore, it is of great social and economic significance to carry out research on the comprehensive utilization of animal bones. [0003] At present, calcium deficiency in the human body has become a global problem, especially in children, the elderly and pregnant women. Therefore, finding an effective calcium supplement product is of great ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/312A23L1/305C12P3/00C12N1/14C12N1/16A23L13/20
Inventor 马俪珍甄润英梁丽雅任小青张建荣
Owner TIANJIN AGRICULTURE COLLEGE
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