Test strip for rapidly detecting glutamic pyruvic transaminase and preparation method

Abstract

The invention relates to a test strip for detecting glutamic-pyruvic transaminase and a preparation method thereof. The technical proposal is as follows: a glass fibre film provided with a gold-labeled anti-ALT antibody A is stuck to a substrate, a sample cushion is stuck to the glass fibre film, absorbent paper is stuck to the other end of the substrate, a coated film on which a detection area and a control area are formed is stuck to the middle of the substrate, the detection area is coated by an anti-ALT antibody B, and the control area is coated by an anti-IgG antibody. The invention avoids the respective disadvantages of manual operation, inhuman element influence to results, easy occurrence of false positive, greater disturbance of endogenic pyruvic acid, demand of special equipment, partial hydrogen peroxide assumption and icterus affection to detection results caused by the easy affection of reducing substances in blood, and the like of a Reitman method, a performance rate method and a microplate pyruvic acid oxidase method. The invention adopts the transaminase quality determination, avoids interference factors in an enzyme vitality test, simplifies the procedure of ALT detection, has easy operation and can be suitable for field use.

Description

technical field [0001] The invention relates to a test strip, in particular to a test strip and a preparation method for detecting alanine aminotransferase in whole blood, serum or plasma by using colloidal gold immunochromatography technology. Background technique [0002] Alanine aminotransferase (ALT) is a non-plasma-specific enzyme that exists freely in the cytoplasm, especially in hepatocytes, and has a large concentration difference between the inside and outside of the cells; When enlargement or cell necrosis, alanine aminotransferase can be released from the cells into the blood, resulting in a significant increase in serum or plasma alanine aminotransferase. Therefore, it is recommended by the World Health Organization as the most sensitive detection index of liver function damage. From the perspective of clinical blood safety, the detection of alanine aminotransferase activity concentration makes up for the missed detection problem of hepatitis virus infection "wi...

AI Technical Summary

Problems solved by technology

Lai’s method is a classic method for this project, but manual operation is required. Quantitative analysis is carried out by a spectrophotometer and the experimental results are manually recorded. It is cumbersome and the results are easily affected by human factors.

Moreover, the determination is greatly affected by the quality of the specimen, such as lipemia, hemolysis, etc., which will cause the increase of alanine aminotransferase to varying degrees, resulting in false positive results of the specimen.

Although the rate method is more accurate, it is greatly interfered by endogenous pyruvate and requires special equipment (automatic or semi-automatic biochemical analyzer). Since the biochemical analyzer is a precision instrument and should not be moved frequently, it cannot be used in mobile blood donation vehicles. It is used on the Internet, and the biochemical instrument is expensive, which cannot be afforded by grassroots blood banks, and is not easy to popularize

Microplate pyruvate oxidase method: It has the advantages of simple operation, fast measurement speed and color comparison with a microplate reader, but it is easily affected by reducing substances in the blood, containers and sampling equipment and consumes part of the hydrogen peroxide, making it The result is low; at the same time, the requirements for the reaction time are strict, and the time of adding the enzyme working solution should be accurate and timely, otherwise the hydrogen peroxide will decompose and the result will be low; in addition, the test result is also greatly affected by jaundice

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