Method for extracting cervus elaphus Linnaeus polysaccharide

An extraction method and polysaccharide extraction technology, applied in the field of red antler polysaccharide preparation, can solve the problems of low activity and low extraction rate of red antler polysaccharide, and achieve high activity, high extraction rate, suitable for popularization and application

Inactive Publication Date: 2009-06-03
NORTHEAST FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems of low extraction rate and low activity of red antler polysaccharide, the present invention provides a method for preparing red antler polysaccharide by strong electric field method, which can effectively improve the extraction rate and product activity of red antler polysaccharide

Method used

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Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0006] Specific embodiment 1: In this specific embodiment, the steps of a method for extracting red deer antler polysaccharides are as follows: 1. Extraction of red deer antler polysaccharides: firstly carry out screening of red deer antler, select fresh red deer antler, and take two-thirds of the apex , sliced, crushed, and the ratio of solid to liquid is 1g: 15mL ~ 1g: 20mL, add velvet antler powder and aqueous solution respectively, extract with a strong electric field for 25 to 35 minutes to obtain an extract containing red antler polysaccharide, and finally use a decanter centrifuge Carry out solid-liquid separation at a speed of 1500-2000r / min, separate the extract and residue, add the residue to the aqueous solution at a ratio of 1g: 15mL to 1g: 20mL, continue strong electric field extraction and centrifugation, and continuously Extract 3 times, remove the residue, and combine the extracts. 2. Concentration: Concentrate the red antler polysaccharide extract with a vacuu...

specific Embodiment approach 2

[0007] Embodiment 2: This embodiment differs from Embodiment 1 in that in step 1, the extraction electric field strength is 60-90 kV per square centimeter, the high-voltage electric field pulses are 600-800 times, and the pulse frequency is 200-300 Hz. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0008] Embodiment 3: This embodiment is different from Embodiment 1 in that it is characterized in that the solid content in the concentrated solution in step 2 is 70% to 80%. Others are the same as in the first embodiment.

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Abstract

A method for extracting cervus elaphus Linnaeus polysaccharide relates to a method for preparing cervus elaphus Linnaeus polysaccharide and solves the problems of low cervus elaphus Linnaeus polysaccharide extracting rate, low activity and complex production process, which exist in a conventional method. The method for extracting cervus elaphus Linnaeus comprises the following steps: selecting, slicing and crushing ervus elaphus Linnaeus, extracting the ervus elaphus Linnaeus by using a strong electric field, separating extract with a supercentrifuge, removing albumen and lipid substances, carrying out concentration by using a vacuum scraper type film, and carrying out freeze-drying by using a vacuum freeze-dryer so as to obtain cervus elaphus Linnaeus polysaccharide freeze-dried powder. The cervus elaphus Linnaeus polysaccharide prepared by the invention is a natural extract, brings no side effect to human body, has the functions of strengthening immunologic function, resisting tumor and inflammation, adjusting lipid, resisting cruor and thrombus, promoting healing of cornea trauma, improving eye dryness, and the like, and can be applicable to the fields such as food, health products, cosmetics, medicine, biochemical industry, etc.

Description

technical field [0001] The invention relates to a preparation method of red deer antler polysaccharide. Background technique [0002] Antler polysaccharide (PAPS) is a special active factor of velvet antler. It is a kind of acidic proteoglycan combined with protein. And improve eye dryness and so on. [0003] At present, red deer antler polysaccharide can be widely used in the production of food, medicine, health care products, and cosmetics. It is a worldwide multifunctional product popular in Europe, America, Japan, and Korea, and its market prospect is very broad. Although there are many studies on velvet antler polysaccharides, the extraction methods of velvet antler polysaccharides are mainly ultrasonic and reflux extraction. A large number of free radicals will be generated during the extraction of velvet antler polysaccharides by ultrasonic method, which will destroy the physiological activity of velvet antler polysaccharides. The method is a high-temperature extrac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 王振宇焦岩
Owner NORTHEAST FORESTRY UNIVERSITY
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