Simple and new method for identifying copy numbers of transgenic plants

A technology of transgenic plants and copy number, applied in the field of molecular detection of transgenic plants, can solve the problems of expensive experimental equipment and consumables, and achieve the effects of increasing the difficulty of the experiment, shortening time-consuming and low-cost.
CN101445826AInactive Publication Date: 2009-06-03新疆农业科学院核技术生物技术研究所
0 Cites 4 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
新疆农业科学院核技术生物技术研究所
Publication Date
2009-06-03
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention provides a simple and new method of using a common PCR cycler and a specific single copy gene as interior labels to realize the identification of copy numbers of transgenic plants. The invention is characterized by extracting the total DNA of young leave samples to be detected, adjusting the concentration of the DNA of all the samples to be consistent, designing specific primers of target genes and interior label genes, further determining the best PCR cycle number on the basis of PCR molecular detection, then making parallel PCR and agarose electrophoresis of the target genes and the interior label genes under the same cycle number and finally determining the copy numbers of the target genes according to the ratio of optical density values of the target genes and the interior genes in an electrophoresis image. The invention has the advantage that the invention is beneficial to screening the transgenic plants with stable expression in transgenic plant breeding; the invention has the advantages of simple operation, short time consumption, low cost, safety and accuracy; and the invention combines the PCR molecular detection of the transgenic plants and causes a laboratory for performing the PCR molecular detection of the transgenic plants to realize batch detection without adding experimental equipment and increasing the difficulty.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to a simple and new method for identifying the copy number of transgenic plants, which belongs to the field of molecular detection of transgenic plants. Background technique

[0002] With the development of plant genetic engineering technology, it is possible to transfer exogenous genes into plants to improve plant yield, quality, stress resistance or bioreactors for certain secondary metabolites. Transgenic plants are playing an increasingly important role in modern agricultural technology. The effect of transgenics depends on the stable expression of exogenous genes in transgenic plants. However, transgenic plants with multiple copies of repeated transgenic sequences often make it impossible for foreign genes to be stably expressed in transgenic plants, or even not expressed at all. This is a phenomenon that easily occurs in transgenic plants. Especially the pollen tube transgenic method is more prone to this phenomenon due t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More