Integrated chip and device thereof, and method for preparing micrometre level dispersoid

Abstract

The invention discloses an integrated chip, a device adopting the integrated chip and a method for preparing micron-sized dispersoid; the substrate of the integrated chip is the substrate with hydrophilic surface; two layers of adjacent structures are a group of structures, and the integrated chip comprises at least one group of structures; in one group of structures, the first structure is provided with M levels of gradients, and the second structure is provided with N levels of gradients; wherein, M and N are natural numbers, and M is less than N; among the gradients, the primary gradient is provided with H1 channel 1, H2 branches are led out from each channel 1 in the secondary gradient, and H1*H2...*HN channel N are formed; one side which is far away from each channel M is provided with a connecting channel respectively; the integrated chip also comprises at least a collecting groove; the integrated chip also comprises at least one collecting groove which is connected with the output end of each collecting channel for collecting and exporting products. The invention has very high productivity and absolute advantage in the aspect of the large scale production of contrast agent.

Description

【Technical field】 [0001] The present invention relates to the application of contrast agent contrast imaging technology, in particular, a large-scale integrated chip using microfluidic technology to manufacture micron-scale dispersions; a device using the chip, which can be used to prepare ultrasonic contrast agents; and The method for preparing the micron-scale dispersion by using the device can be used as an ultrasound contrast agent. 【Background technique】 [0002] Noninvasive, real-time, and dynamic detection of structural and functional information about tissues has become increasingly important in clinical diagnosis. Ultrasonic diagnostic technology not only has the above-mentioned characteristics, but also has the advantages of safety, wide adaptability, repeated inspection, strong ability to identify soft tissues, strong flexibility and low price compared with X-ray and MRI technologies. It is the most widely used imaging technology in the world. [0003] The appli...

AI Technical Summary

Problems solved by technology

[0016] However, the vibroacoustic method has the following disadvantages: (1) The process parameters of the probe-type vibro-acoustic instrument, including power, position of the probe on the liquid surface, depth, etc., are not easy to control, and the process reproducibility is affected to a certain extent; (2) due to the It must be placed inside the preparation solution, so it is difficult to perform aseptic operation during the acoustic vibration process, and there is a possibility of metal contamination, which adds a certain degree of difficulty to the quality control and preparation process of the contrast agent; (3) More heat, the activity of lipids, especially when preparing contrast agents carrying certain ligands, drugs or genes, has a great impact on the activity of ligands, drugs or genes that are vibrating at the same time

[0018] However, the mechanical oscillation method has the following disadvantages: (1) the particle size of the microbubbles of the contrast agent cannot be precisely controlled; (2) the particle size distribution of the formed microbubbles is relatively wide, and the acoustic properties are unstable. Microbubbles of 10 microns, these large microbubbles may cause blockage or rupture of local blood vessels, making the use of ultrasound contrast agents potentially risky; (3) the shell thickness of the microbubbles is not uniform; (4) mechanical Oscillation method is used to generate sufficient force to introduce gas from the surrounding environment into the liquid solution to form bubbles. The oscillation speed largely determines the number and size of microbubbles formed. For example, SonoVue, a contrast agent approved for clinical use in China, uses a manual method preparation, the repeatability is not good enough

Images