Extraction process for Hericium erinaceus polysaccharide
An extraction process, Hericium erinaceus polysaccharide technology, applied in the field of Hericium erinaceus polysaccharide extraction process, can solve the problems of long extraction time, high energy consumption, low yield, etc. Effect
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Embodiment 1
[0016] 1) Material pretreatment: select 20 g of Hericium erinaceus fruiting bodies, crush them, pass through a 30-mesh sieve, add 300 ml of water to soak for 1 hour, and wait for extraction;
[0017] 2) Microwave extraction: adjust the power of the microwave generator to 300w, carry out microwave treatment on the above-mentioned pretreated extraction material for 8 minutes, filter, carry out solid-liquid separation, and prepare to concentrate the supernatant after discarding the solid;
[0018] 3) Concentration: put the collected supernatant in a rotary evaporator, concentrate under reduced pressure at 40°C, and concentrate to 1 / 4 of the original volume;
[0019] 4) Alcohol precipitation: Stir and mix the concentrated solution and 70% edible ethanol at a ratio of 1:3. Since the polysaccharide is insoluble in ethanol, a precipitate is formed and precipitates at the base of the container;
[0020] 5) Centrifugation: After the concentrated solution-ethanol mixture was placed in t...
Embodiment 2
[0024] 1) Material pretreatment: select 20 g of Hericium erinaceus fruiting bodies, crush them, pass through a 40-mesh sieve, add 400 ml of water to soak for 2 hours, and wait for extraction;
[0025] 2) Microwave extraction: the power of the microwave generator is selected as 450w, and the above-mentioned pretreated extraction material is subjected to microwave treatment for 6 minutes, centrifuged, and solid-liquid separation is performed, and the supernatant is prepared to be concentrated after discarding the solid;
[0026] 3) Concentration: put the collected supernatant in a rotary evaporator, concentrate under reduced pressure at 55°C, and concentrate to 1 / 4 of the original volume;
[0027] 4) Alcohol precipitation: Stir and mix the concentrated solution and 95% edible ethanol at a ratio of 1:1.5. Since the polysaccharide is insoluble in ethanol, a precipitate is formed and precipitates at the base of the container;
[0028] 5) Centrifugation: After the concentrated solut...
Embodiment 3
[0032] 1) Material pretreatment: select 20 g of Hericium erinaceus fruiting bodies, crush them, pass through a 60-mesh sieve, add 500 ml of water to soak for 4 hours, and wait for extraction;
[0033] 2) Microwave extraction: the power of the microwave generator is selected as 600w, and the above-mentioned pretreated extraction material is subjected to microwave treatment for 4 minutes, filtered, and solid-liquid separation is performed, and the supernatant is concentrated after discarding the solid;
[0034] 3) Concentration: put the collected supernatant in a rotary evaporator, concentrate under reduced pressure at 70°C, and concentrate to 1 / 4 of the original volume;
[0035] 4) Alcohol precipitation: Stir and mix according to the ratio of 1:1 between the concentrated solution and 100% edible ethanol. Since the polysaccharide is insoluble in ethanol, a precipitate is formed and precipitates at the base of the container;
[0036] 5) Centrifugation: After the concentrated solu...
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