Method for biosynthesis of 15N mark helix alga

Abstract

The invention relates to a method for biosynthesizing <15>N marked spirulina and a culture medium for realizing the method. The culture medium comprises a carbon source, a nitrogen source, a phosphorus source, inorganic salt, and trace elements; moreover, <15>N urea is used as the nitrogen source; and dextrose and sodium bicarbonate are used as the carbon source. The method of the invention comprises enriching and acclimating the species of the <15>N spirulina, inoculating the species of the <15>N spirulina in the culture medium for culturing to obtain culture liquid containing the <15>N marked spirulina; and separating the <15>N marked spirulina from the culture liquid. The method also comprises recycling of the culture liquid. The method can obtain the <15>N marked spirulina with high abundance ratio; moreover, the yield is high and the cost is low.

Description

technical field [0001] The invention belongs to the field of biology or algae cultivation, more specifically, the invention relates to a 15 Biosynthetic method of N-labeled spirulina. Background technique [0002] Spirulina (Spirulina) belongs to Cyanophyta, Cyanophyceae, Oscillatoriaceae, Seglophytes, and Spirulina. It is a multicellular filamentous aquatic microalgal plant. It is named for its spiral shape under the microscope. There are more than 35 species of algae, currently the main ones used for production are Spirulina platensis and Spirulina maxima. Spirulina is rich in high-quality protein, multivitamins, minerals, chlorophyll, γ-linolenic acid and other unsaturated fatty acids and β-carotene, polysaccharides, phycobiliproteins, trace elements and other substances that have special medicinal effects on the human body. The protein content of active substances is about 60-70% of the total cells. The nutrients contained in spirulina are not only rich, high-quality, ...

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Problems solved by technology

The patent application number is 98124744.X, the patent name is "a method for domesticating and eating fresh spirulina", and the patent application number is 200510005016.2, the patent name is "a kind of indoor cultivation of spirulina and its control of oxygen release and ecological landscaping The method of ", discloses the species of family breeding spirulina, breeding environment conditions and breeding containers, etc., these two schemes are simple and practical, can artificially control the temperature and light conditions, carry out continuous cultivation and harvest for a period of time and directly replace the nutrient solution, and The nutrient solution is not recycled, and the algae used are common blunt-topped or extremely large spirulina, and the spirulina produced is of natural abundance, which is not suitable for 15 Cultivation of N-labeled Spirulina

The patent application number is 200610048617.6, and the patent name is "Spirulina Wastewater Treatment Process", which discloses a physical and chemical treatment method for wastewater after Spirulina cultivation. This method requires special equipment and pipelines, consumes water, electricity, chemicals, etc., and is not suitable for 15 Treatment of N-labeled Spirulina culture waste

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