Efficient disease-resistant phosphate solubilizing bacterial manure for tobacco and production method thereof
A production method and technology of phosphate-solubilizing bacteria, which can be applied to bacteria, organic fertilizers, etc., can solve problems such as abnormal plant production, and achieve the effects of inhibiting the infection of bacteria and viruses, increasing the proportion of high-quality smoke, and reducing costs.
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[0034] The screening of Pseudomonas aeruginosa XQ902, a disease-resistant phosphate solubilizing bacteria strain of the present invention, is described below.
[0035] 1. Primary screening
[0036] (1) Medium glucose 5-10g, NaCl 0.2-0.5g, KCl 0.2-0.5g, MgSO 4 ·7H 2 O 0.2—0.5g, (NH 4 ) 2 SO 4 0.2—0.5g, FeSO 4 ·7H 2 O0.02-0.05g, MnSO 4 ·H 2 O 0.02-0.05g, Ca3(PO 3 ) 4 2.0-5.0g, distilled water 1000ml, pH7.0~7.5.
[0037] (2) Screening method
[0038] The plate dilution separation method was used to select colonies with fast growth and large hydrolytic transparent circles, and after separation and purification by streaking, they were stored on the slant.
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