Valveless micro-flow control gradient real-time reaction chip and reaction control method

Abstract

The invention relates to a valveless micro-fluidic gradient real-time reaction chip and a reaction control method in the micro-fluidic technical field. Magnetic particles are marked on target molecules which need reacting with drugs, while non-retentive micro-column arrays are arranged in a concentration gradient field forming zone in the front of the tail end of an outlet of a traditional pyramid-type micro-fluidic gradient chip, by the structure, a high gradient magnetic field generated under an external magnetic field previously adsorbs drug molecules marked with the magnetic particles before the gradient field reaction, thereby the drug molecules are fixed in a pipeline at the front end of the outlet of the pyramid-type micro-fluidic gradient chip. When in gradient real-time reaction, after being continuously shunted and mixed and finally forming the gradient field at the tail end of the chip and flowing through the soft non-retentive micro-column arrays, drugs can simultaneously react with the drug molecules fixed hereof. The design of the chip is mainly used in various aspects such as the research of biological macromolecule reaction and action mechanism, the screen of drugs, and the like.

Description

technical field [0001] The invention relates to a chip and a control method in the field of microfluidic technology, in particular to a valveless microfluidic gradient real-time reaction chip and a reaction control method. Background technique [0002] With the continuous development of biomedicine and chemistry, many studies are currently focused on the molecular level. In recent years, the emergence of microfluidic technology has led to a new direction in biochemical analysis. Microfluidic technology refers to the technology of manipulating and analyzing fluids in micron-scale (mainly width and height) pipes. Compared with conventional methods, microfluidic technology can effectively reduce reagent consumption, shorten reaction time, make automation higher, achieve high-throughput, large-scale detection, thereby reducing costs and more reliable detection results. Therefore, it has been widely used in disease detection, environmental monitoring, chemical synthesis, gene s...

AI Technical Summary

Problems solved by technology

Although Hsin-Chih Yeh's design can effectively combine the formation of gradient field and its reaction with target molecules, this type of chip uses external valves to control or fix the reactive biomolecules in the chip, which requires a multi-layer structure and increases processing. difficulty

The opening and closing of the valve requires an additional air source to operate it, which increases the difficulty of control

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