Production technology of pig brain protolysate

A technology of protein hydrolyzate and production process, which is applied in the field of biochemical raw materials, can solve the problems of high production cost and cumbersome operation, and achieve the effects of reducing production cost, simplifying process links and improving fat removal rate

Inactive Publication Date: 2009-09-09
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are some deficiencies in individual links, such as secondary degreasing in the degreasing link, which is cumbersome to

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Take 9 parts of fresh healthy porcine pancreas with fat and connective tissue removed, add 1 part of fresh pineapple peel after washing and decontamination, homogenate, and refrigerate for later use;

[0017] Take 100 kg of fresh and healthy pig brains from which surface mucous membranes and blood stains have been removed, add 100 L each of acetone and n-butanol after homogenization, stir and degrease for 4 hours, and then suction filter, the resulting filter residue is vacuum-dried at 50°C to obtain 83.2 kg of degreased brain powder, add 600L of 0.1mol / L calcium chloride solution, adjust the pH value to 7.5 with 0.5mol / L HCl, then add 16L of the homogenized mixture of fresh healthy pig pancreas and fresh pineapple peel prepared in advance, and stir at 35°C Hydrolyzed for 8 hours, boiled for 10 minutes after hydrolysis, cooled to room temperature, suction filtered, concentrated the obtained filtrate under reduced pressure, and then spray-dried the obtained concentrated s...

Embodiment 2

[0019] Take 9 parts of fresh healthy porcine pancreas with fat and connective tissue removed, add 1 part of fresh pineapple peel after washing and decontamination, homogenate, and refrigerate for later use;

[0020] Take 80 kg of fresh and healthy pig brains from which surface mucous membranes and blood stains have been removed, add 40 L of acetone and 120 L of n-butanol after homogenization, stir and degrease for 6 hours, then suction filter, and vacuum-dry the resulting filter residue at 50°C to obtain 58.0 kg of degreased brain powder, add 0.1 mol / L calcium chloride solution 450L, adjust the pH value to 7.5 with 0.5mol / L HCl, then add the pre-prepared fresh healthy porcine pancreas and fresh pineapple peel homogenized mixture 10L, stir and hydrolyze at 37°C for 10 After hydrolysis, boil for 10 minutes, cool to room temperature and then suction filter, concentrate the obtained filtrate under reduced pressure, then spray-dry the obtained concentrate at 50°C to obtain 33.1kg o...

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PUM

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Abstract

The invention provides a production technology of pig brain protolysate. The technology comprises two main courses: (1) a fresh and healthy pig brain is subjected to procedures like decreasing to obtain decreased brain powders, (2) the decreased brain powders are subjected to procedures like hydrolysis to obtain the product. Compared with the prior art, the invention simplifies the technologic processes, changes the current secondary decreasing method into the dual-degreaser primary decreasing method, and improves the decreasing rate to further strengthen the degree of the hydrolysis; the mixture, which is obtained by homogenating inexpensive, fresh and healthy pig pancreas and fresh pineapple peels, is used for replacing high-priced commercial enzyme used for hydrolysis. Production cost is reduced while the quality of the product is guaranteed.

Description

technical field [0001] The invention belongs to the production technology of biochemical raw materials. Background technique [0002] Cerebroprotein hydrolyzate is a raw material drug for oral use obtained by degreasing and enzymatically hydrolyzing healthy pig brains. The main components are a mixture of various low-molecular-weight active peptides and various amino acids. It has a remarkable effect on the treatment of brain functional diseases. The current production process of pig brain protein hydrolyzate is as follows: fresh pig brain removes enveloped blood stains, etc., homogenizes, adds 4 times the volume of acetone to degrease, separates and obtains a precipitate, adds an equal amount of ether to the precipitate, washes and degreases for 1 hour, and then filters , vacuum drying to obtain defatted brain powder; after the defatted brain powder is dissolved in water, it is hydrolyzed with pepsin and trypsin in turn, the hydrolyzed solution is boiled, centrifuged and f...

Claims

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Application Information

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IPC IPC(8): A61K35/30A61P25/00C12P21/02
Inventor 孙中武王宏伟
Owner NORTHEAST FORESTRY UNIVERSITY
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