Corn protein high F value oligopeptide production method

A technology of corn protein and production method, which is applied in protein food processing, protein food ingredients, food science, etc., can solve the problems of low enzymatic hydrolysis efficiency, cumbersome operation, chemical pollution, etc., achieve good selectivity, increase hydrolysis degree, and easy The effect of enzymatic decomposition

Active Publication Date: 2007-05-23
吉林省长吉图开发建设现代农业投资有限公司
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Zein is insoluble in water, if it is not solubilized by appropriate methods, and it is directly used for enzymatic hydrolysis to produce high F value oligopeptides, the enzymatic hydrolysis efficiency will be extremely low
Traditionally, reducing agents, acid, alkali, heat treatment and other solubilization methods are used, which are cumbersome to operate and easy to cause chemical pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] 1. Degreasing and deodorizing treatment of corn yellow powder

[0015] Subjecting maize flour to supercritical CO 2 Fluid extraction, extraction pressure 10MPa, temperature 35°C, time 0.5 hours, CO 2 The flow rate is 15L / hour.

[0016] 2. Extraction of Zein

[0017] with supercritical CO 2 The maize flour after fluid extraction is used as a raw material, and the ethanol concentration is 60%, the mass ratio of maize flour and ethanol solution is 1:8, the extraction temperature is 50° C., and the pH value is 8.0. Extract for 2 hours to obtain a zein solution. Centrifuge the zein solution at a speed of 3000 rpm for 5 minutes to obtain a supernatant, dilute the supernatant with distilled water until the concentration of ethanol is 30% and when turbidity occurs, adjust the pH value to 6.5 with 4mol HCI solution, add A saturated sodium chloride solution with a total volume of 2% promotes the precipitation of prolamin, and centrifuges at a speed of 4000rpm for 5 minutes to...

Embodiment 2

[0027] 1. Degreasing and deodorizing treatment of corn yellow powder

[0028] Subjecting maize flour to supercritical CO 2 Fluid extraction, extraction pressure 20MPa, temperature 45°C, time 1 hour, CO 2 The flow rate is 20L / hour.

[0029] 2. Extraction of Zein

[0030] with supercritical CO 2The maize flour after fluid extraction is used as a raw material, and the ethanol concentration is 70%, the mass ratio of maize flour and ethanol solution is 1:9, the extraction temperature is 55° C., and the pH value is 8.5. Extract for 2 hours to obtain a zein solution. Below with embodiment 1.

[0031] 3. Moisturizing treatment

[0032] Add an appropriate amount of drinking water at 25°C to the zein powder, stir evenly, and let it stand for 15 minutes to make the zein powder fully absorb water and ensure its water content is 20%.

[0033] 4. High temperature and high pressure extrusion treatment

[0034] Preheat the twin-screw extruder and set the temperature at 130°C. When the ...

Embodiment 3

[0040] 1. Degreasing and deodorizing treatment of corn yellow powder

[0041] Subjecting maize flour to supercritical CO 2 Fluid extraction, extraction pressure 30MPa, temperature 45°C, time 1.5 hours, CO 2 The flow rate is 25L / hour.

[0042] 2. Extraction of Zein

[0043] with supercritical CO 2 The maize flour after fluid extraction is used as a raw material, and the ethanol concentration is 80%, the mass ratio of maize flour and ethanol solution is 1:10, the extraction temperature is 60° C., and the pH value is 9.0, and extraction is performed for 2 hours to obtain a zein solution. Below with embodiment 1.

[0044] 3. Moisturizing treatment

[0045] Add an appropriate amount of drinking water at 25°C to the zein powder, stir evenly, and let it stand for 15 minutes to make the zein powder fully absorb water and ensure that its water content is at 25%.

[0046] 4. High temperature and high pressure extrusion treatment

[0047] Preheat the twin-screw extruder and set th...

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PUM

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Abstract

The preparation method for high-H-value oligopeptide from zein comprises, using corn powder as material, hypercritical CO2 liquid extracting to remove fat and smelly materials in said powder and extract zein; adding some water to the zein; taking extruding and modifying treatment at given temperature and pressure to increase its solubility; using two proleases for two-step enzymolysis to zein at given substrate concentration, enzyme concentration, pH value, and time condition; then, separating, purifying, drying, smashing and obtaining the product with F value more than 20.

Description

technical field [0001] The invention belongs to health food processing technology. Background technique [0002] In the amino acid mixture, the molar ratio of branched chain amino acid (BCAA for short) to aromatic amino acid (AAA for short) is called Fiscer, or F value for short. High F value oligopeptide is a low molecular weight physiologically active peptide with special physiological functions formed after protease acts on protein. It is a nutritional supplement and food ingredient for patients with liver diseases. Modern medical and nutritional studies have proved that high F-value oligopeptides can inhibit hepatic encephalopathy, improve the protein nutritional status of liver patients, and improve the body's anti-fatigue ability. It is reported that in 1990, Shin-ya T. et al. degraded zein with basophilic protease and actinase to develop a non-bitter high F-value oligopeptide with a yield of 56%, an F value of 20.47, and an AAA content of 1.86. %. In 1992, Shuji A....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/00A23J1/12A23J3/34
Inventor 王大为张艳荣
Owner 吉林省长吉图开发建设现代农业投资有限公司
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