Powder for pig sperm dilution and room temperature preservation and preparation method and application thereof

A technology for storing pig semen at room temperature, applied in the field of artificial insemination of livestock, to achieve good preservation effect, simple ingredients, and prolonged storage time

Active Publication Date: 2009-09-30
佛山市多乐畜牧器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since pig semen is generally only stored for 2 to 3 days at r

Method used

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  • Powder for pig sperm dilution and room temperature preservation and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0034] Example 1 According to the diluent powder formula given in Table 1, prepare the raw materials:

[0035] Table 1 Diluent powder formula

[0036] Reagent content glucose 27g

[0037] Sodium bicarbonate 1.0g Sodium citrate 6.0g EDTA 2.15g Citric acid 2.8g Tris 5.5g Hepes 4.0g penicillin 0.6g Streptomycin 1g

[0038] Various chemicals (analytical grade) in the purchased formula, large particles or agglomerated ingredients need to be crushed and sieved through an 80-mesh sieve to form powder.

[0039] Accurately weigh the various ingredients, mix them according to the proportions in the formula, and stir them thoroughly, then pack them into bags, vacuum and seal to obtain a pack of pig semen dilution powder. The above process generally requires the temperature to be controlled at 18~26℃. The relative humidity is controlled at 45-65%.

[0040] Under normal circumstances, the dilution should be prepared...

Example Embodiment

[0045] Example 2 According to the diluent powder formula given in Table 2, prepare the raw materials:

[0046] Table 2 Diluent powder formula

[0047] Reagent content glucose 26.0g Sodium bicarbonate 2.0g Sodium citrate 8.0g EDTA 2.00g Citric acid 3.5g Tris 5.0g Hepes 2.0g penicillin 0.8g Chloramphenicol 0.8g

[0048] Other operations are the same as in Example 1. Half an hour before the semen dilution, the diluent is prepared. A pack of pig semen diluent powder is dissolved to 1000ml in double distilled distilled water, and continuously stirred with a magnetic stirrer until it is completely dissolved. Adjust the pH to 7.0 and heat to 37°C. According to sperm motility and density, adjust the dilution ratio, and slowly add the dilution into the semen.

Example Embodiment

[0049] Example 3 According to the diluent powder formula given in Table 3, prepare the raw materials:

[0050] Table 3 Diluent powder formula

[0051] Reagent content glucose 30.0g Sodium bicarbonate 0.8g Sodium citrate 6.0g EDTA 3.00g Citric acid 2.0g Tris 6.0g Hepes 2.35g penicillin 1.0g Streptomycin 0.5g

[0052] Other operations are the same as in Example 1. Half an hour before the semen dilution, the diluent should be prepared. A pack of pig semen diluent powder is dissolved in double distilled water to 1000ml, and the magnetic stirrer is continuously stirred until it is completely dissolved. The pH is adjusted to 6.9 and heated to 37°C. . According to sperm motility and density, adjust the dilution ratio, and slowly add the dilution into the semen.

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PUM

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Abstract

The invention discloses a powder for pig sperm dilution and room temperature preservation and the preparation method and the application thereof. The powder for pig sperm dilution and room temperature preservation comprises the following components according to the weight portion: 26.0-30.0 of glucose, 0.8-2.0 of sodium bicarbonate, 6.0-8.0 of sodium citrate, 2.0-3.0 of EDTA, 2.5-3.5 of citric acid, 5.0-6.0 of Tris, 2.0-4.0 of Hepes and disinfectant. Diluent with pH value being 6.8-7.0 is obtained after the powder is dissolved by distilled water. The powder is applied to room temperature long-term pig sperm preservation or common or high-time pig sperm dilution; when the pig sperm is stored under 17 DEG C, the activity of the pig sperm is more than 0.7 after 5 days. The invention has simple prescription component, low cost, long pig sperm preservation time and good preservation effect and can effectively prolong the preservation time of the pig sperm, enlarge the utilization efficiency of a superior breed boar and meet the demands of pig raising.

Description

technical field [0001] The invention belongs to the technical field of livestock artificial insemination, and in particular relates to a pig semen dilution and room temperature preservation powder, a preparation method thereof and an application in low-temperature long-term preservation and dilution of pig semen. Background technique [0002] Pig is an important part of animal husbandry and the main source of meat consumption in my country, accounting for about 70-80% of the total meat consumption. China's pig stock and per capita pork production rank first in the world, but there is a big gap between my country's per capita pork consumption level and developed countries. my country's pig industry is of great significance to the construction of the national economy and the improvement of people's living standards. The fecundity of pigs has an important influence on the production efficiency of pig industry. Therefore, there are continuous efforts to increase the fertility ...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 张守全朱健彬
Owner 佛山市多乐畜牧器械有限公司
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