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Powder for pig sperm dilution and room temperature preservation and preparation method and application thereof

A technology for storing pig semen at room temperature, applied in the field of artificial insemination of livestock, to achieve good preservation effect, simple ingredients, and prolonged storage time

Active Publication Date: 2009-09-30
佛山市多乐畜牧器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since pig semen is generally only stored for 2 to 3 days at room temperature, it is difficult to implement the above policy

Method used

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  • Powder for pig sperm dilution and room temperature preservation and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 Prepare raw materials according to the dilution powder formula given in Table 1:

[0035] Table 1 Dilution powder formula

[0036] Reagent content glucose 27g

[0037] sodium bicarbonate 1.0g Sodium citrate 6.0g EDTA 2.15g citric acid 2.8g Tris 5.5g Hepes 4.0g penicillin 0.6g streptomycin 1g

[0038] Various chemicals (analytically pure) in the purchased formula, large particles or components that have been agglomerated need to be crushed and sieved with an 80-mesh sieve to form a powder.

[0039] Accurately weigh various ingredients, mix according to the ratio in the formula, and fully stir, then pack into bags, vacuumize and seal to obtain a pack of pig semen dilution powder. The above process generally requires the temperature to be controlled at 18-26°C. The relative humidity is controlled at 45-65%.

[0040] Under normal circumstances, the dilution should be prepared half ...

Embodiment 2

[0045] Embodiment 2 Prepare raw materials according to the dilution powder formula given in Table 2:

[0046] Table 2 Diluent powder formula

[0047] Reagent content glucose 26.0g sodium bicarbonate 2.0g Sodium citrate 8.0g EDTA 2.00g citric acid 3.5g Tris 5.0g Hepes 2.0g penicillin 0.8g Chloramphenicol 0.8g

[0048] Other operations are the same as in Example 1. Half an hour before the semen dilution, the diluent was prepared. A pack of porcine semen dilution powder was dissolved in double-distilled water to 1000ml, stirred continuously by a magnetic stirrer until completely dissolved, adjusted to a pH value of 7.0, and heated to 37°C. According to the sperm viability and density, adjust the dilution ratio, and slowly add the diluent to the semen.

Embodiment 3

[0049] Embodiment 3 Prepare raw materials according to the dilution powder formula given in Table 3:

[0050] Table 3 Dilution powder formula

[0051] Reagent content glucose 30.0g sodium bicarbonate 0.8g Sodium citrate 6.0g EDTA 3.00g citric acid 2.0g Tris 6.0g Hepes 2.35g penicillin 1.0g streptomycin 0.5g

[0052] Other operations are the same as in Example 1. The diluent should be prepared half an hour before semen dilution. A pack of pig semen dilution powder is dissolved in double-distilled water to 1000ml, stirred continuously by a magnetic stirrer until completely dissolved, adjusted to a pH of 6.9, and heated to 37°C . According to the sperm viability and density, adjust the dilution ratio, and slowly add the diluent to the semen.

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PUM

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Abstract

The invention discloses a powder for pig sperm dilution and room temperature preservation and the preparation method and the application thereof. The powder for pig sperm dilution and room temperature preservation comprises the following components according to the weight portion: 26.0-30.0 of glucose, 0.8-2.0 of sodium bicarbonate, 6.0-8.0 of sodium citrate, 2.0-3.0 of EDTA, 2.5-3.5 of citric acid, 5.0-6.0 of Tris, 2.0-4.0 of Hepes and disinfectant. Diluent with pH value being 6.8-7.0 is obtained after the powder is dissolved by distilled water. The powder is applied to room temperature long-term pig sperm preservation or common or high-time pig sperm dilution; when the pig sperm is stored under 17 DEG C, the activity of the pig sperm is more than 0.7 after 5 days. The invention has simple prescription component, low cost, long pig sperm preservation time and good preservation effect and can effectively prolong the preservation time of the pig sperm, enlarge the utilization efficiency of a superior breed boar and meet the demands of pig raising.

Description

technical field [0001] The invention belongs to the technical field of livestock artificial insemination, and in particular relates to a pig semen dilution and room temperature preservation powder, a preparation method thereof and an application in low-temperature long-term preservation and dilution of pig semen. Background technique [0002] Pig is an important part of animal husbandry and the main source of meat consumption in my country, accounting for about 70-80% of the total meat consumption. China's pig stock and per capita pork production rank first in the world, but there is a big gap between my country's per capita pork consumption level and developed countries. my country's pig industry is of great significance to the construction of the national economy and the improvement of people's living standards. The fecundity of pigs has an important influence on the production efficiency of pig industry. Therefore, there are continuous efforts to increase the fertility ...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 张守全朱健彬
Owner 佛山市多乐畜牧器械有限公司
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