Preparation method of encoding microspheres

A technology for encoding microspheres and microspheres, applied in the field of biomedicine, can solve the problem of insufficiency of encoding capacity, etc., and achieve the effects of facilitating implementation, increasing capacity and reducing cost

Inactive Publication Date: 2011-07-27
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Abstract
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Problems solved by technology

However, the encoding capacity of only 100 is obviously unable to meet the needs of modern biomedical research, and its detection method is done by expensive flow cytometer

Method used

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  • Preparation method of encoding microspheres
  • Preparation method of encoding microspheres
  • Preparation method of encoding microspheres

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Embodiment Construction

[0024] The steps of the method for preparing the encoded microspheres of the present invention and their beneficial effects will be further described in detail below in conjunction with several specific embodiments of the present invention and accompanying drawings:

[0025] The invention realizes the encoding function of the microsphere by preparing the microsphere with the micropattern through the microprocessing technology. Through a series of processes such as growth, deposition, etching, etc., microspheres are prepared on silicon wafers or other substrates, and through release, the microspheres are finally suspended in the solution. Describe the preparation method of the present invention below with the compound of silicon dioxide and silicon nitride as microsphere material, as figure 1 and figure 2 The flow chart of preparation method and process schematic diagram are as shown in:

[0026] Firstly, a first layer of photoresist 2 with a thickness of 1 μm is coated on a...

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Abstract

The invention discloses a preparation method of encoding microspheres, comprising the steps of coating a sacrifice layer on an underlay, growing or preparing a microsphere material layer compounded by a single layer or multiple layers in a depositing mode on the surface of the sacrifice layer, carrying out semiconductor microprocessing procedures of photoresistive spread coating, photodevelopment, corrosion and cleaning for the microsphere material layer to produce an encoding micro pattern or array, carrying out photoetching again according to the required microsphere size of the preparationand cleaning to remove the microsphere material layer outside the microsphere outline, selecting a liquid capable of dissolving the sacrifice layer, submersing the underlay with the sacrifice layer inside and the encoding microsphere into the liquid, and finally releasing the microspheres differently encoded to suspend in the liquid. The invention realizes the preparation of the encoding micropheres through a microprocessing technology to improve the capacity of the microsphere encoding and can adopt a general fluorescence microscope to detect so as to effectively reduce the cost for biological analysis; in addition, the preparation process has better repeatability and is conveniently actualized.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for preparing microspheres for encoding by processing micropatterns. Background technique [0002] Suspension array (suspension array), also known as liquid array (liquid array), its technical principle is different from solid-phase biochip technology [Note 1, Note 2]. The core of suspension array technology is to use a variety of distinguishable microspheres, In other words, the microspheres can be encoded and decoded during detection. Microsphere chip technology is an emerging protein chip technology and a new multifunctional liquid-phase chip analysis platform developed in recent years. This technology organically integrates technologies such as colored microsphere technology, laser technology, applied fluidics, high-speed digital signal processor and computer algorithm, and has quite high detection specificity and sensitivity. Usually used in immunoassays, accounting stu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J13/02
Inventor 李炯鲍芳郑克孝曹榕
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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