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Method for hormone-free scale culture and production of dendrobium officinale embryo dry product

A hormone-free technology of Dendrobium candidum, which is applied in the field of preparation of dried germ products of Dendrobium candidum, can solve problems such as resource shortage of Dendrobium candidum, achieve low cost, improve growth status, and solve the effects of resource shortage

Active Publication Date: 2009-10-21
SHANGHAI TANGZHENSHENG BIO TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a method for large-scale cultivation without hormones to produce dried germs of Dendrobium candidum. The problem of shortage of resources

Method used

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  • Method for hormone-free scale culture and production of dendrobium officinale embryo dry product
  • Method for hormone-free scale culture and production of dendrobium officinale embryo dry product
  • Method for hormone-free scale culture and production of dendrobium officinale embryo dry product

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Experimental program
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Embodiment 1

[0031] (1) Preparation of explants, culture medium production and inoculation:

[0032] (1) choose long to have 3-5 leaf, stem thick 0.3-0.5 adult dendrobium officinale strain body;

[0033] (2) explant sterilization procedure:

[0034] After the explants are collected, put them in a 250ml small triangular flask, add 1-2 drops of detergent, and rinse them repeatedly with tap water until there is no foam. Follow the steps below to enter the sterilization process on the ultra-clean table: soak in 50% ethanol for 3-5 seconds → 20% "84" solution for 7-8 minutes → 0.1% mercury solution for 12-15 minutes → rinse with sterile water for several minutes After several times, put it on the water-permeable sterile paper to absorb the attached water;

[0035] (3) Medium production and preparation:

[0036] Choose "MS" basic ingredients, use 1 / 2 macroelements + iron whole + micro whole + full organic conventional + sucrose 30g / L+100g banana juice 10% / L+agar powder 4.8g / L pH5.8, conventio...

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Abstract

The invention relates to a method for hormone-free scale culture and production of a dendrobium officinale embryo dry product, which comprises the following steps: (1) culture and inoculation of an explant; (2) subculturing multiplication of an embryoid; (3) hormone-free first-order liquid culture and screening; (4) hormone-free second-order aerated culture; (5) hormone-free ultimate 20-liter aerated culture; and (6) post treatment of a fresh finished product. The method has the advantages of simple steps, short production cycle, low cost, environmental protection and suitability for industrial production, and solves the problem of dendrobium officinale resource shortage.

Description

technical field [0001] The invention belongs to the field of preparation of dried germs of dendrobium candidum, in particular to a method for producing dried germs of dendrobium candidum through large-scale cultivation without hormone. Background technique [0002] As a traditional precious Chinese herbal medicine, Dendrobium has always been valued by the medical community, and is highly praised by medical scientists because of its special curative effect. Dendrobium is relatively harsh on the production environment, with slow growth and extremely low biological yield. Coupled with long-term unrestrained excavation by people and the deterioration of the ecological environment, the entire medicinal Dendrobium is facing an endangered situation. Among them, Dendrobium candidum, also known as Dendrobium Huoshanense (Dictionary of Traditional Chinese Medicine 1985) is excellent. [0003] For the dried germ of Dendrobium officinale Kimura et Migo, no hormone-free large-scale cult...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A61K36/8984
Inventor 郭建荣陶艳汤佳静
Owner SHANGHAI TANGZHENSHENG BIO TECH
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