Anti-tumor pharmaceutical composition and preparation method thereof

A production method and composition technology, applied in the field of biopharmaceuticals, can solve problems such as not very obvious effects, and achieve the effects of reasonable design, enhanced immunity, and high extraction purity

Active Publication Date: 2011-06-15
ZHEJIANG FANGGE PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are also some drugs on the market that enhance the body's own immunity, but many drugs have serious side effects, and their effects are not very obvious

Method used

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  • Anti-tumor pharmaceutical composition and preparation method thereof
  • Anti-tumor pharmaceutical composition and preparation method thereof
  • Anti-tumor pharmaceutical composition and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Preparation of Antrodia Antrodia Extract: Take Antrodia Antrodia Coarse Powder, add 75% ethanol at a concentration of 6 times the weight of Antrodia Antrodia Cottae Coarse Powder, and filter after extracting for 2 hours at a temperature of 75°C; Add 75% ethanol which is 4 times the weight of the filter residue, extract at a temperature of 75°C for 1.5 hours, then filter; combine the two filtrates, reflux and condense the ethanol in an ethanol reflux tower until there is no alcohol smell, and obtain an extract; Add water 22 times the weight of the filter residue to the above-mentioned filter residue after the second filtration, and filter after 2 hours of extraction; take the filter residue after filtration, add water 10 times the weight of the filter residue to the filter residue, and filter after 1.5 hours of extraction; merge twice After the filtrate and the above extract, carry out evaporation and concentration, and concentrate to a density of 1.02g / cm 3 , and th...

experiment example

[0038] 1. Drug evaluation index: tumor inhibition rate (%).

[0039]

[0040] Observation time: 14 days.

[0041] 2. The main steps of the experiment:

[0042] 2.1 Tumor strain and solid tumor transplantation method: The mouse liver cancer (H22) tumor strain was routinely preserved by the Central Laboratory of Cancer Institute, Chinese Academy of Medical Sciences. The tumor cells were taken out from the liquid nitrogen, quickly thawed and revived at 37°C, and inoculated into the abdominal cavity of mice. After 8 days, the abdomen of the mice was swollen, and the tumor was successfully revived.

[0043] 2.2 Tumor inoculation and grouping: the tumor-bearing mice were sacrificed, ascites cancer cells were extracted under aseptic conditions, diluted, counted, and each mouse was inoculated with 0.2 ml of cell suspension. Randomly divided into 6 groups, 10 in each group. That is, normal saline group, cyclophosphamide injection group, Antrodia 1 group, 2 groups, 3 groups, 4 gro...

Embodiment 2

[0058] (1) Preparation of Antrodia Antrodia Extract: Take Antrodia Antrodia Coarse Powder, add 60% ethanol with a concentration of 10 times the weight of Antrodia Antrodia Cottae Coarse Powder, and filter after extracting for 3 hours at a temperature of 60° C.; Add 60% ethanol with a concentration 5 times the weight of the filter residue, extract at a temperature of 65° C. for 2 hours, and then filter; combine the two filtrates, reflux and condense the ethanol in an ethanol reflux tower until there is no alcohol smell, and obtain an extract; Add water 15 times the weight of the filter residue to the filter residue after the above second filtration, and filter after 3 hours of extraction; take the filter residue after filtration, add water 15 times the weight of the filter residue to the filter residue, and filter after 3 hours of extraction; merge twice After the filtrate and the above-mentioned extract, carry out evaporation and concentration, and concentrate to a density of 1...

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Abstract

The invention relates to an anti-tumor pharmaceutical composition and a preparation method thereof, and belongs to the technical field of bio-pharmaceuticals. The pharmaceutical composition comprises the following components: 20 to 60 percent of extract of brown heart rot of cinnamomum kanehirai, 5 to 30 percent of glossy ganoderma extract and 20 to 60 percent of grifola frondosa extract. The preparation method comprises the following steps: preparing the extract of the brown heart rot of the cinnamomum kanehirai; preparing the glossy ganoderma extract; preparing the grifola frondosa extract;and mixing the extract of the brown heart rot of the cinnamomum kanehira, the glossy ganoderma extract and the grifola frondosa extract. The anti-tumor pharmaceutical composition is an immunopotentiator, has no toxic and side effect, and mainly achieves the anti-tumor effect by reinforcing the immunological competence of the human body. The anti-tumor pharmaceutical composition has reasonable compatibility, and all the components are from natural products, so that the anti-tumor pharmaceutical composition is safe and has no toxic and side effect. The tumor inhibition rate of the anti-tumor pharmaceutical composition is 26.16 percent (P is less than 0.05) and is equivalent to that of the prior anti-cancer medicine cyclophosphamide, but the toxicity of the anti-tumor pharmaceutical composition is obviously lower than that of the cyclophosphamide. The preparation method for the anti-tumor pharmaceutical composition has reasonable design, simple and convenient operation and high extractionpurity.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and in particular relates to an antitumor pharmaceutical composition and a production method thereof. Background technique [0002] Antrodia cinnamomea is also called Antrodia cinnamomea or Antrodia camphorata, and its scientific name is Antrodia Camphorata. [0003] Antrodia camphorata is a unique fungus in Taiwan, which only grows on the unique camphor tree in Taiwan. Antrodia Cinnamomea grows in the decayed hollow interior of the trunk of Cinnamomum camphora or on the moist surface of a fallen trunk. The wild Antrodia camphorata grows on old camphor trees. The fruiting bodies vary in shape, such as plate-shaped, bell-shaped, horseshoe-shaped or tower-shaped; they are bright red when they are born, and gradually become white, light reddish brown, light brown or light yellowish brown. Antrodia camphorata gas fragrance is hard, flat. It has the effects of dispelling wind and promoti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/074A61P35/00
Inventor 徐财泉茆广华周晓芬
Owner ZHEJIANG FANGGE PHARMA
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