Process combination for extracting polysaccharide in golden mushroom mycelium cells and determination method thereof

A technology of Flammulina velutipes and intracellular polysaccharides, applied in the field of biopharmaceutical production, can solve problems such as increased production costs and difficult product separation, and achieve the effects of increasing yield, improving separation efficiency, and shortening extraction time

Inactive Publication Date: 2009-10-21
蒋海军
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Problems solved by technology

However, since the enzyme extraction will increase the production cost and also bring difficulties to the separation of the product, the amount of addition should be strictly controlled

Method used

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  • Process combination for extracting polysaccharide in golden mushroom mycelium cells and determination method thereof

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Embodiment Construction

[0022] Zhejiang Province Qingyuan County Fangge Medicinal Mushroom Co., Ltd. produces Flammulina velutipes mycelium dry powder polysaccharide extraction process combination, and its composition is: solid-liquid ratio is 1: 30 by mass ratio, and compound enzyme addition is 2%, and ultrasonic power is 200W, enzymolysis time 50min, enzymolysis temperature 55°C, enzymolysis pH 5.0.

[0023] A method for determining the combination of processes for extracting intracellular polysaccharides from Flammulina velutipes mycelium is:

[0024] 1) The dry powder of Flammulina velutipes mycelium produced by Fangge Medicinal Fungi Company in Qingyuan County, Zhejiang Province was selected as the raw material for extraction.

[0025] 2) Determine the ratio of material to liquid

[0026] Polysaccharides were extracted by hot water extraction. Select 100g of mycelium dry powder from step 1), take the water addition multiple as the analysis factor, set 10, 20, 30, 40, 50, 60 times (mass multipl...

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Abstract

The invention relates to a process combination for extracting polysaccharide in golden mushroom mycelium cells. The solid-liquid ratio according to weight is 1: 10-1: 60, the adding amount of complex enzyme accounts for 0.5 percent to 5.0 percent of the weight of the raw materials, the range of ultrasonic power is 100w to 500w, the range of enzymolysis time is 40min to 100min, the range of enzymolysis temperature is 30 DEG C to 60 DEG C, and the range of enzymolysis pH value is 4.0 to 8.0. The determination method of the process combination for extracting polysaccharide in golden mushroom mycelium cells is as follows: high-quality mycelium is selected to be deeply fermented, thus obtaining mycelium dry powder; the solid-liquid ratio is determined; the adding amount of complex enzyme is determined; and the optimal extracting process combination for extracting mycelium dry powder polysaccharide in an ultrasonic-complex enzyme method is determined.

Description

technical field [0001] The invention relates to biopharmaceutical production technology, in particular to a combination of techniques for extracting intracellular polysaccharides from Flammulina velutipes mycelium and a determination method thereof. Background technique [0002] Flammulina velutipes is an edible fungus cultivated in autumn, winter and early spring. It is famous for its smooth and tender cap, crisp handle, rich nutrition and delicious taste. According to the determination, the amino acid content of Flammulina velutipes is very rich, higher than that of common mushrooms, especially the content of lysine is particularly high, and lysine has the function of promoting children's intellectual development. Flammulina velutipes dry product contains 8.87% protein, 60.2% carbohydrate, and 7.4% crude fiber. Regular consumption can prevent and treat ulcer disease. Recent research shows again that a kind of material contained in Flammulina velutipes has good anticancer ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12S3/02
Inventor 蒋海军
Owner 蒋海军
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