Method for culturing microalgae by illumination way and reactor thereof

A technology of light culture and photobioreactor, applied in the field of combined photobioreactor, can solve the problems of difficult temperature control, insufficient light, low carbon dioxide utilization rate, etc., to avoid insufficient light, overcome temperature and pH uncontrollable effects

Inactive Publication Date: 2009-11-11
UNIV OF SCI & TECH BEIJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Closed photobioreactors include pipeline type, flat plate type, columnar airlift type, stirred fermenter, floating film bag, etc. Compared with open culture tanks and runways, although there are some differences in carbon dioxide fixation efficien...

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  • Method for culturing microalgae by illumination way and reactor thereof
  • Method for culturing microalgae by illumination way and reactor thereof
  • Method for culturing microalgae by illumination way and reactor thereof

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Embodiment Construction

[0015] 1. Medium for selection and cultivation of algal species:

[0016] The algal species used in the experiment of the present invention is a prokaryotic chlorella-USTB01 (Chlorella sp) screened by ourselves with dual functions of autotrophy and heterotrophy, strain preservation number: cgmcc No1448, preservation date: August 25, 2005 day. The composition of the medium is as follows (1000ml deionized water): MgSO 4 7H 2 O 1.0g, Na 2 HPO 4 12H 2 O 2.0g, KNO 3 2.0g, CaCl 2 30mg, EDTA-2Na 20mg, ferric ammonium citrate 10mg. The initial pH of this prepared medium was 8.0.

[0017] 2. Fermentation tank and pipeline combined photobioreactor:

[0018] The combined photobioreactor used in the present invention is mainly composed of a fermentation tank and pipelines connected in series, and the chlorella USTB-01 culture circulates in the fermentation tank and pipelines through a pump. The volume of the fully self-controlled fermentation tank used is 50 liters, and four...

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Abstract

The invention relates to a method for culturing microalgae by an illumination way and a reactor thereof, which belong to the technical field of biology. In the invention, carbon dioxide is injected into a pipeline through a carbon dioxide feeding device during the microalgae culturing process, and microalgae liquid culture from a fermentation tank and the injected carbon dioxide are fully mixed through a circulating pump and then flow in the pipeline; as the pipeline is twisted on a cylindrical light source, the carbon dioxide can be fully utilized for photosynthesize; and the microalgae culture that flows out from the pipeline enters the fermentation tank, then is fully mixed with aerated air by stirring of a stirring vane, and then pumped into the pipeline again to form flow circulation. The bioreactor adopting the method can absorb 40-80 percent of carbon dioxide in fixed aerated air under the condition of injecting 1-40 percent of carbon dioxide, obtains the microalgae with dry cell weight concentration up to more than 3.5g/L after five-day culture and achieves the purposes of effectively absorbing carbon dioxide and culturing the microalgae.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular provides a combined photobioreactor in which a fermenter and pipelines are connected in series, and is used for efficiently fixing carbon dioxide and cultivating microalgae. Background technique [0002] As the simplest photosynthetic organism, microalgae have survived on the earth for more than two billion years. Through photosynthesis, they have laid an important material foundation for the production of oxygen in the earth's early atmosphere and the origin and development of animals. It is reported that more than 90% of the oxygen in the atmosphere is produced by microalgae, and the photosynthesis efficiency of microalgae is 5 to 10 times that of ordinary plants. Therefore, microalgae play a major role in fixing carbon dioxide in the atmosphere. With the advancement of science and technology, the advantages of microalgae, such as fast growth, high photosynthetic efficiency, and s...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12M1/42C12M1/36C12M1/06C12R1/89
CPCC12M21/02C12M27/02
Inventor 闫海贾璇景建克王子敬杨帅
Owner UNIV OF SCI & TECH BEIJING
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