Rapid propagation and sugar-free rooting culture method and application of apple rootstock malus prunifolia

Abstract

The invention belongs to the technical field of apple seedling propagation, and discloses a rapid propagation and sugar-free rooting culture method and application of apple rootstock malus prunifolia.The rapid propagation and sugar-free rooting culture method comprises the following steps of collecting malus prunifolia branches, carrying out water culture for about 15 days, taking grown tender shoots as explant materials, carrying out disinfection, inoculating into a primary culture medium, and culturing in an illumination environment; after seedling division, inoculating the seedlings into asubculture medium, and continuously culturing in an illumination environment; after growing for 30-40 days, selecting malus prunifolia seedlings which grow robustly, transferring the malus prunifoliaseedlings into a sugar-free culture box, placing the sugar-free culture box on a special culture shelf for sugar-free culture, introducing carbon dioxide gas, and carrying out sugar-free rooting culture for 25-35 days in an illumination environment to emerge seedlings. The culture medium is not prone to pollution, under the condition that gas exchange with the outside is guaranteed, photosynthesis of plants is fully utilized, the rooting rate and the transplanting survival rate of malus prunifolia tissue culture seedlings are increased, rooting and seedling hardening are combined, the time cost is saved, and high-quality and efficient production of the malus prunifolia tissue culture seedlings can be accelerated.

Description

technical field [0001] The invention belongs to the technical field of apple seedling propagation, and in particular relates to a rapid propagation and sugar-free rooting cultivation method and application of apple rootstock catalpa chinensis. Background technique [0002] At present, the breeding of seedlings in my country is mainly based on layering and tissue culture, but there are many problems in the traditional tissue culture technology. The medium used for traditional tissue culture rooting is mostly sugar-containing organic substances, which lead to the serious growth of microorganisms such as bacteria and fungi, causing pollution; because the medium contains agar and other substances, the medium is too dense, which is not conducive to root respiration of tissue culture seedlings, and the rooting effect is poor. Poor; and in order to reduce the pollution rate, the internal space of the tissue culture bottle used in traditional tissue culture is small, the humidity is...

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Problems solved by technology

[0005] In summary, the problems in the prior art are: the traditional tissue culture technology has the disadvantages of easily polluting the culture dish, poor rooting of seedlings, low survival rate of transplanting in the later stage, and high cost; common dwarf rootstocks are not suitable for soil, climate Planting in areas with poorer conditions; the growth of the traditional seed-propagated catalpa is different after the garden is established, and the garden appearance is uneven, which brings a lot of inconvenience to the follow-up management and correspondingly increases the cost.

[0006] The difficulty of solving the above-mentioned technical problems: the medium used for traditional tissue culture rooting is mostly sugar-containing organic substances, which are very easy to pollute. In order to reduce the pollution rate, culture bottles with less internal space have to be used, which will lead to the growth of tissue culture seedlings. The humidity of the internal growth environment is high, there is basically no gas exchange with the outside world, the growth of the seedlings is weak, and the rooting is poor

Therefore, if the problem of easy pollution of traditional tissue culture rooting medium is

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