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Method for preparing polygonatum odoratum polysaccharide

Polygonatum polysaccharides and Polygonatum polysaccharide technology, applied in the field of Polygonatum polysaccharide preparation, can solve the problems of high content of protein, pigment and small molecular substances, difficulty in filtering the extract, high extraction temperature, etc., achieve low polysaccharide loss rate, improve product quality The effect of purity and short production cycle

Inactive Publication Date: 2009-11-25
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a new method for preparing polysaccharides from Polygonatum polysaccharides, which can maintain the integrity of Polygonatum Polygonatum traditional Chinese medicine, whose industrial production yield can reach more than 28%, and whose polysaccharide content can reach more than 90%. The method can overcome the problems of high extraction temperature, cumbersome operation, time-consuming, and difficult filtration of the extract in the heating and reflux extraction method. Problems such as high content of pigments and small molecular substances

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] 1) Ultrasonic extraction: cut Polygonatum Polygonatum into small pieces of 0.3-1cm, add 12 times the amount of water, soak for 20 minutes, ultrasonically extract for 20 minutes, pass the extract through a 200-mesh nylon screen, and then centrifuge; the obtained residue is added to 10 times the weight of the residue in water, soak for 20 minutes, ultrasonically extract for 20 minutes, and then centrifuge; combine the two extracts, then centrifuge, and concentrate the supernatant under reduced pressure to 1 / 6 of the combined volume of the two extracts;

[0015] 2) Alcohol precipitation: Slowly stir and add absolute ethanol to the above concentrated solution. When the ethanol content reaches 95%, adjust the pH to 7, let it stand for 4 hours, separate the precipitate, wash it with 95% ethanol three times, discard Wash with alcohol, collect the precipitate, and dry it in vacuum.

[0016] 3) Deproteinization: dissolve the alcohol precipitate with 10 times of water, preheat in...

Embodiment 2

[0021] Step 1) In the ultrasonic extraction process, add 16 times the amount of water to the small pieces of Polygonatum Polygonatum, soak for 20 minutes, then ultrasonically extract for 20 minutes, add 12 times the weight of the residue to the obtained residue, soak for 20 minutes, and then ultrasonically extract for 20 minutes.

[0022] Step 2) In the alcohol precipitation process, let stand for 8 hours.

[0023] Step 2) In the deproteinization process, 8 times of water is used to dissolve the alcohol precipitate, and the amount of papain is 1.0% (g / ml) of the solution volume; the addition of trichloroacetic acid is 6% (ml / ml) of the solution volume.

[0024] Step 4) In the decolorization process, LSD208 macroporous adsorption resin is used for the decolorization resin, and the resin is washed with pure water equivalent to 5 times the volume of the liquid entering the column.

[0025] Step 5) In the concentration and drying process, the concentration conditions are: vacuum 0...

Embodiment 3

[0028] Step 1) In the ultrasonic extraction process, add 20 times the amount of water to the small pieces of Polygonatum Polygonatum, soak for 20 minutes, then ultrasonically extract for 20 minutes, and add water 16 times the weight of the residue to the obtained residue, soak for 20 minutes, and then ultrasonically extract for 20 minutes.

[0029] Step 2) In the alcohol precipitation process, let stand for 5h.

[0030] Step 2) In the deproteinization process, use 5 times of water to dissolve the alcohol precipitate, and the amount of papain is 0.8% (g / ml) of the solution volume; the addition of trichloroacetic acid is 4% (ml / ml) of the solution volume.

[0031] Step 4) In the decolorization process, LSI 296 macroporous adsorption resin is used for the decolorization resin, and the resin is washed with pure water equivalent to 4 times the volume of the liquid entering the column.

[0032] Step 5) In the concentration and drying process, the concentration conditions are: vacuum...

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Abstract

The invention discloses a method for preparing polygonatum odoratum polysaccharide, which prepares a polygonatum odoratum polysaccharide product by ultrasonic extraction, deposition in alcohol, deproteinization by zymohydrolysis, decolorization by macroporous adsorption resin column chromatography, concentration and vacuum drying. The yield of the method is over 28 percent. The polysaccharide content of the polygonatum odoratum polysaccharide product can reach over 90 percent.

Description

technical field [0001] The invention relates to a preparation method of traditional Chinese medicine polysaccharide, in particular to a preparation method of polygonatum polysaccharide. Background technique [0002] Polygonatum Polysaccharide is an active ingredient extracted from the rhizome of Polygonatum Polygonatum, a traditional Chinese medicine plant. It contains odoratan and 4 kinds of Polygonatum-fructan-O-A, B, C, D. The Polygonatum Polysaccharide content in Polygonatum Polygonatum is about 6-12%. The Polygonatum Polysaccharide can be made into various dosage forms by adding appropriate auxiliary materials through pharmaceutical methods. Polygonatum polysaccharide pharmaceutical preparations can lower blood sugar and blood lipids in patients with hyperlipidemia and hyperglycemia. At present, the preparation method of polygonatum polysaccharide mainly involves the water extraction process. "Chinese Pharmacopoeia" (2005 edition) adopts the heating reflux extraction ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P3/10A61P3/06C12S3/02
Inventor 王冬梅张京芳李登武杨秀平李雪艳
Owner NORTHWEST A & F UNIV
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