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Fe reducing bacteria-mineral complex inocula capable of accelerating degradation of organic chloride and preparation method thereof

A technology of organic chlorine and minerals, applied in the field of iron-reducing bacteria-mineral composite bacterial agent and its preparation, to achieve the effects of environmental friendliness and harmlessness, easy large-scale production, and simple production methods

Inactive Publication Date: 2009-11-25
GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to literature and patent searches, no report has been found in the prior art on the use of iron-reducing bacteria and iron-containing minerals to prepare composite bacterial agents and apply them to organochlorine pollution remediation

Method used

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  • Fe reducing bacteria-mineral complex inocula capable of accelerating degradation of organic chloride and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Enterobacter aerogenes-ferrihydrite composite bacterial agent

[0030] (1) culture medium

[0031] Select beef extract peptone medium, 5g / L beef extract, 10g / L peptone, 5g / LNaCl, add defoaming agent foam enemy by 0.05% by weight, adjust pH to 7.05, fully dissolve, after stirring evenly, put in seed tank or Sterilize at 121°C for 30 minutes under 15 lbs of pressure in the fermenter, then cool to 35°C for later use.

[0032] (2) shake flask culture

[0033] Pick a ring from the slant culture medium of normal preserved Enterobacter aerogenes (E. aerogenes CGMCC 1969) and inoculate it into a Erlenmeyer flask containing beef extract peptone liquid medium for shaking flask culture. The conditions are: the temperature is 35° C., the rotating speed is 200 rpm, the volume of liquid in the triangular flask is 40%, and the incubation time is 9 hours.

[0034] (3) Seed tank fermentation

[0035] According to the inoculum amount of 2%, the Enterobacter aerogenes shake ...

Embodiment 2

[0045] Example 2 Enterobacter aerogenes-red soil colloidal composite bacterial agent

[0046] (1) culture medium

[0047] Select beef extract peptone medium, 5g / L beef extract, 10g / L peptone, 5g / LNaCl, add antifoaming agent polyether antifoaming agent GPE according to 0.05% by weight, adjust pH to 7.17, fully dissolve, stir evenly, Sterilize in a seed tank or a fermenter at 121°C for 40 minutes under 15 pounds of pressure, and cool to 30°C for use.

[0048] (2) shake flask culture

[0049] Pick a ring from the slant culture medium of normal preserved Enterobacter aerogenes (E. aerogenes CGMCC 1969) and inoculate it into a Erlenmeyer flask containing beef extract peptone liquid medium for shaking flask culture. The conditions are as follows: the temperature is 37°C, the rotation speed is 220 rpm, the liquid volume in the Erlenmeyer flask is 40%, and the incubation time is 10 hours.

[0050] (3) Seed tank fermentation

[0051] According to the inoculum amount of 3%, the shak...

Embodiment 3

[0059] Example 3 Comamonas iron-reducing-goethite composite bacterial agent

[0060] (1) culture medium

[0061] Select beef extract peptone medium, 5g / L beef extract, 10g / L peptone, 5g / L NaCl, add defoamer soybean oil at a weight ratio of 0.05%, adjust the pH to 7.12, fully dissolve, stir evenly, and put it in the seed tank or ferment The tank was sterilized at 121°C for 30 minutes under 15 pounds of pressure, and cooled to 37°C for use.

[0062] (2) shake flask culture

[0063] Pick a ring from the slant culture medium of Comamonas ferrireducens (CGMCC 1968) preserved normally and inoculate it into a Erlenmeyer flask containing beef extract peptone liquid medium for shaking flask culture. The conditions are as follows: a temperature of 30° C., a rotation speed of 180 rpm, a liquid volume of 40% in the triangular flask, and a culture time of 8 hours.

[0064] (3) Seed tank fermentation

[0065] According to the inoculum amount of 3%, the shake flask bacterial liquid of Co...

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Abstract

The invention discloses a Fe reducing bacteria-mineral complex inocula capable of accelerating degradation of organic chloride and a preparation method thereof. Normal bacteria culture medium is used as a main nutrient, Fe reducing bacterial strain is grown and proliferated in condition of keeping proper pH, temperature and ventilatory capacity, and then obtained fermentation liquor is mixed with iron-bearing mineral in a certain proportion to produce Fe reducing bacteria-mineral complex inocula. (1) Fe reducing bacteria-mineral complex inocula can accelerate degradation of organic chloride and can be one novel processing approach for pollution repairing organic chloride. (2) Fe reducing bacteria is obtained by separating and purifying in natural environment and iron-bearing mineral is original material in natural environment, therefore the invention generates from natural environment and is applied to natural environment, not destroying ecological structure of original natural environment. (3) The production method is simple and easy for mass production.

Description

[0001] The present invention is a divisional application, the filing date of the parent case is June 1, 2007, the application number is 200710028391.8, and the title of the invention is "An iron-reducing bacteria-mineral composite bacterial agent capable of accelerating organic chlorine degradation and its preparation method " technical field [0002] The invention relates to the technical field of organic chlorine degradation, in particular to an iron-reducing bacteria-mineral composite bacterial agent capable of accelerating organic chlorine degradation and a preparation method thereof. Background technique [0003] Organochlorine refers to chlorinated hydrocarbons or aromatic compounds containing one or more chlorine atoms, and is an important class of persistent organic pollutants (POPs), including: DDT (dichloro-diphenyl-trichloroethane, dichlorodiphenyl-trichloroethane Chloroethane), HCH (hexachlorocyclohexane, hexachlorocyclohexane), PCP (pentachlorophenol, pentachlor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C22B3/18A62D3/02B09C1/10C12R1/01A62D101/22
CPCY02P10/20
Inventor 李芳柏周顺桂李晓敏王跃强
Owner GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI