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Ferrum reduction bacterium-mineral composite agent capable of accelerating organic chlorine degradation and preparation method thereof

A compound bacterial agent and organochlorine technology, which is applied in the direction of bacteria and process efficiency improvement, to achieve the effects of being environmentally friendly and harmless, accelerating the degradation of organochlorine, and facilitating large-scale production

Inactive Publication Date: 2008-01-02
GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to literature and patent searches, no report has been found in the prior art on the use of iron-reducing bacteria and iron-containing minerals to prepare composite bacterial agents and apply them to organochlorine pollution remediation

Method used

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  • Ferrum reduction bacterium-mineral composite agent capable of accelerating organic chlorine degradation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Enterobacter aerogenes-ferrihydrite composite bacterial agent

[0029] (1) culture medium

[0030] Select beef extract peptone medium, 5g / L beef extract, 10g / L peptone, 5g / LNaCl, add defoaming agent foam enemy by 0.05% by weight, adjust pH to 7.05, fully dissolve, after stirring evenly, put in seed tank or Sterilize at 121°C for 30 minutes under 15 lbs of pressure in the fermenter, then cool to 35°C for later use.

[0031] (2) shake flask culture

[0032] Pick a ring from the slant culture medium of normal preserved Enterobacter aerogenes (E. aerogenes CGMCC 1969) and inoculate it into a Erlenmeyer flask containing beef extract peptone liquid medium for shaking flask culture. The conditions are: the temperature is 35° C., the rotating speed is 200 rpm, the volume of liquid in the triangular flask is 40%, and the incubation time is 9 hours.

[0033] (3) Seed tank fermentation

[0034] According to the inoculum amount of 2%, the Enterobacter aerogenes shake ...

Embodiment 2

[0044] Example 2 Enterobacter aerogenes-red soil colloidal composite bacterial agent

[0045] (1) culture medium

[0046] Select beef extract peptone medium, 5g / L beef extract, 10g / L peptone, 5g / LNaCl, add antifoaming agent polyether antifoaming agent GPE according to 0.05% by weight, adjust pH to 7.17, fully dissolve, stir evenly, Sterilize in a seed tank or a fermenter at 121°C for 40 minutes under 15 pounds of pressure, and cool to 30°C for use.

[0047] (2) shake flask culture

[0048] Pick a ring from the slant culture medium of normal preserved Enterobacter aerogenes (E. aerogenes CGMCC 1969) and inoculate it into a Erlenmeyer flask containing beef extract peptone liquid medium for shaking flask culture. The conditions are as follows: the temperature is 37°C, the rotation speed is 220 rpm, the liquid volume in the Erlenmeyer flask is 40%, and the incubation time is 10 hours.

[0049] (3) Seed tank fermentation

[0050] According to the inoculum amount of 3%, the shak...

Embodiment 3

[0058] Example 3 Comamonas iron-reducing-goethite composite bacterial agent

[0059] (1) culture medium

[0060] Select beef extract peptone medium, 5g / L beef extract, 10g / L peptone, 5g / L NaCl, add defoamer soybean oil at a weight ratio of 0.05%, adjust the pH to 7.12, fully dissolve, stir evenly, and put it in the seed tank or ferment The tank was sterilized at 121°C for 30 minutes under 15 pounds of pressure, and cooled to 37°C for use.

[0061] (2) shake flask culture

[0062] Pick a ring from the slant culture medium of Comamonas ferrireducens (CGMCC 1968) preserved normally and inoculate it into a Erlenmeyer flask containing beef extract peptone liquid medium for shaking flask culture. The conditions are as follows: a temperature of 30° C., a rotation speed of 180 rpm, a liquid volume of 40% in the triangular flask, and a culture time of 8 hours.

[0063] (3) Seed tank fermentation

[0064] According to the inoculum amount of 3%, the shake flask bacterial liquid of Co...

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Abstract

The invention discloses an iron reduced bacterial-mineral composite bacteriostatic which can accelerate to degrade organic chlorine and the preparing method. The invention regards the general bacterial culture medium as the main nutrient, which makes the strain with the reduced iron active grow and breed at proper PH, temperature and venting condition, prepares the reduced iron bacterial-mineral composite bacteriostatic by mixing the fermentation liquor and the mineral with iron. The iron reduced bacterial-mineral composite bacteriostatic can accelerate the effect of the organic chlorine degradation, which provides a new path for restoring the pollution of the organic chlorine. The iron reduced bacterial is got by separating and purifying from the natural environment and the mineral with iron is from the natural environment, so the invention doesn't damage the ecological system structure. The production method is simple and is fit for the mass production.

Description

technical field [0001] The invention relates to the technical field of organic chlorine degradation, in particular to an iron-reducing bacteria-mineral composite bacterial agent capable of accelerating organic chlorine degradation and a preparation method thereof. Background technique [0002] Organochlorine refers to chlorinated hydrocarbons or aromatic compounds containing one or more chlorine atoms, and is an important class of persistent organic pollutants (POPs), including: DDT (dichloro-diphenyl-trichloroethane, dichlorodiphenyl-trichloroethane Chloroethane), HCH (hexachlorocyclohexane, hexachlorocyclohexane), PCP (pentachlorophenol, pentachlorophenol), PCBs (Polychlorinated biphenyls, polychlorinated biphenyls), etc. Most organochlorines are strongly hydrophobic, bioaccumulative and refractory, and some have endocrine disrupting effects, which are very harmful to the environment and organisms. Therefore, organochlorine pollution and its remediation have become a rese...

Claims

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Application Information

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IPC IPC(8): C12N1/20C22B3/18
CPCY02P10/20
Inventor 李芳柏周顺桂李晓敏王跃强
Owner GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI