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Method for culturing autologous tissue fibroblast

A technology of fibroblasts, culture methods, applied in the field of tissue materials

Inactive Publication Date: 2011-03-16
杭州安倍生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

And patients of different ages have different anti-wrinkle effects and satisfaction with postoperative surgery. Young patients have shallow and few wrinkles, strong autologous cell activity, more proliferation and differentiation, and better anti-wrinkle effects; poor

Method used

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  • Method for culturing autologous tissue fibroblast
  • Method for culturing autologous tissue fibroblast

Examples

Experimental program
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Embodiment Construction

[0061] The skin specimen that cuts under aseptic condition is put into immediately and is equipped with basal culture fluid (DMEM low-sugar culture fluid, contains 20% (the ratio of the present invention all refers to percentage by weight, the same below) fetal bovine serum, 100U / ml penicillin and 100U / ml streptomycin) in centrifuge tubes, transported to the laboratory, placed in a 4°C refrigerator, and processed skin samples within 24 hours.

[0062] 2 Fibroblast culture

[0063] Equipment and materials:

[0064] Ultra-clean workbench: It is a kind of laminar flow local air purification equipment. It adopts an adjustable air volume fan system to blow the air out through the "super filter" composed of special microporous foam sheets, forming a continuous The dust-free and sterile ultra-clean air laminar flow, the so-called "high-efficiency special air", removes dust, fungi and bacterial spores larger than 0.3μm. The flow rate of ultra-clean air is 24-30m / min, which is enoug...

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Abstract

The invention relates to a method for culturing autologous tissue fibroblast, which is characterized by orderly comprising a skin specimen treatment step and a fibroblast culture step, wherein the fibroblast culture step comprises a primary fibroblast culture step; and a fibroblast subculture step, a fibroblast cryopreservation step and a fibroblast unfreezing resuscitation step can be set according to actual needs. The method massively cultures the autologous tissue fibroblast by culture in vitro and amplification of autologous fibroblast; and the autologous tissue fibroblast is used for reinfusion treatment on skin collagen defective disease or degenerative disease simply and conveniently with good safety, and does not generate rejection reaction and has obvious effect when used.

Description

Technical field: [0001] The present invention relates to a living tissue material constructed from functional cells in vitro, which is used for implanting in living organisms, repairing or replacing diseased tissues, and restoring the shape, structure and function of tissues and organs; or implanting some Biologically active substances such as hyaluronidase, collagen, tissue stem cells, etc. induce autologous tissue regeneration and achieve autologous tissue fibroblast culture methods to repair tissue and organ functions. Background technique: [0002] With the development of our country's society and economy, the medical cosmetology market is growing day by day. Cosmetic plastic surgery has become one of the fastest-growing subjects in our country. With the rapid development of medical biotechnology, cell biology and molecular biology products continue to appear in The field of cosmetic surgery. Tissue engineering is a new branch of discipline that emerged after the rapid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/08A01N1/02
Inventor 王培科吕烨赵可佳
Owner 杭州安倍生物科技有限公司
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