Deep-sea fish skin collagen oligopeptide with function of reducing blood pressure and preparation method thereof
A collagen oligopeptide and deep-sea fish skin technology, which is applied in the field of food engineering and functional food, can solve the problems of low oligopeptide content and failure to reflect product quality, and achieve good safety and lower blood pressure.
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Embodiment 1
[0029] 1) Thaw the frozen deep-sea fish skin out of the cold storage, disperse it into strips or flakes, put it into the enzymatic hydrolysis pot, add 6 times the amount of water, stir slowly, and raise the temperature to 56°C.
[0030] 2) Adjust the pH to 6.0, add 0.15% fish skin compound protease, control the temperature at 53-56° C., and maintain it for 6 hours.
[0031] 3) After the enzymatic hydrolysis time is up, the temperature is raised to 85°C and maintained for 15 minutes to inactivate the enzyme;
[0032] 4) Add 2% activated carbon to the enzymolysis solution after deactivation, stir for 30 minutes, then add 2% diatomaceous earth and stir slowly for 20 minutes, put the enzymolysis solution into a centrifuge for coarse filtration, and then carry out the coarse filtrate obtained Plate and frame filter for fine filtration to obtain a clear and transparent fine filtrate;
[0033] 5) The fine filtrate obtained is intercepted with a nanofiltration membrane with a molecul...
Embodiment 2
[0038] 1) Thaw the frozen deep-sea fish skin out of the cold storage, grind it with a meat grinder, put it into the enzymatic hydrolysis pot, add 6 times the amount of water, stir and mix, and heat up to 58°C.
[0039] 2) Adjust the pH to 6.0-7.0, add 0.1% compound protease in the amount of fish skin, control the temperature at 55-58°C, add 0.5% compound protease after 3 hours, and continue to maintain for 2 hours.
[0040] 3) After the enzymolysis is finished, add 2% activated carbon, heat up to 85°C while stirring, and maintain it for 20 minutes to inactivate the enzyme, then add 2% diatomaceous earth and stir slowly for 20 minutes, put the enzymolysis solution into a centrifuge for further processing Coarse filtration, and the obtained coarse filtrate is subjected to fine filtration with a plate and frame filter to obtain a clear and transparent fine filtrate.
[0041] 4) The fine filtrate obtained is intercepted with a nanofiltration membrane with a molecular weight cut-of...
Embodiment 3
[0046] 1) Thaw the frozen deep-sea fish skin out of the cold storage, disperse it into strips or flakes, put it into the enzymatic hydrolysis pot, add 8 times the amount of water, stir slowly, and raise the temperature to 56°C.
[0047] 2) Adjust the pH to 6.8, add 0.2% fish skin compound protease, control the temperature at 53-56° C., and keep it for 5 hours.
[0048] 3) After the enzymatic hydrolysis is terminated, the temperature is raised to 85°C and maintained for 20 minutes to inactivate the enzyme;
[0049] 4) Cool down the enzymolysis solution after deactivation to 28-35°C, add 0.1% deodorizing agent, stir for 30 minutes, then add 2% diatomaceous earth and stir slowly for 20 minutes, put the enzymolysis solution into a centrifuge for coarse filtration , the crude filtrate obtained is subjected to plate and frame filter fine filtration again to obtain a clear and transparent fine filtrate;
[0050] 5) The fine filtrate obtained is intercepted with a nanofiltration memb...
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