Capillary electrophoresis test system applied to soil solution cation analysis

A technology of capillary electrophoresis and soil solution, which is applied in the direction of material analysis, material analysis by electromagnetic means, and measurement devices, and can solve problems such as complex components, high concentration of individual components, and detection

Inactive Publication Date: 2009-12-16
INST OF SOIL SCI CHINESE ACAD OF SCI
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Problems solved by technology

However, the current screening system not only cannot be applied to the detection of high-concentration samples, but also is not suitable for the situation where the concentration of components is complex, and the concentration of individual ions exists at the same time, and it is not suitable for equal-proportion dilution detection.
[0005] Therefore, the detection process of traditional detection technology is cumbersom...

Method used

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  • Capillary electrophoresis test system applied to soil solution cation analysis
  • Capillary electrophoresis test system applied to soil solution cation analysis
  • Capillary electrophoresis test system applied to soil solution cation analysis

Examples

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Embodiment 1

[0022] Capillary electrophoresis separation detection system suitable for the composition and concentration of cations in soil solution, containing imidazole 5-10mM, HIBA concentration in buffer solution 8-9mM, 18-crown-6 concentration 2-5mM, the pH value of the detection system is 3.5-4.0. The pH value of the detection system is preferably 3.7. Imidazole is preferably 9 mM. The concentration of HIBA is preferably 8 mM.

Embodiment 2

[0024] Buffer configuration:

[0025] All the materials used are of superior grade, so the water is sterilized secondary deionized water. Prepare a clean 1000mL storage bottle, and accurately weigh 0.6127g of imidazole reagent, 0.8328ga-HIBA reagent, and 1.05736g of 18-crown-6 reagent into the storage bottle with a ten-thousandth balance, and put the prepared 1L Sterilized water was slowly poured into the liquid storage bottle and stirred on a stirrer until the reagents were completely melted, and the liquid storage bottle was covered with a cap. After thorough stirring, the pH was adjusted to 3.7 with 1N HCl to obtain the electrophoresis buffer. This buffer can be used directly for electrophoretic analysis.

[0026] Capillary Electrophoresis Conditions:

[0027] The separation mode is capillary zone electrophoresis (CZE), the detection wavelength is 214nm, pressure injection is used, the condition is 0.2psi×5", the 48-well sample plate is automatically injected, the separat...

Embodiment 3

[0029] The conclusions of Example 2 on rhizosphere determine the components of the buffer solution, and this example reports the correlation study of the application of the buffer solution to standard solutions with different concentrations. Considering that the concentration ratio difference between the components in the sample to be tested is relatively large, and the Ca 2+ The concentration is at a high level, and the H1 to H100 standard solution series are respectively configured according to Table 1.

[0030] For the comprehensive detection of cations, a company provides a broad-spectrum detection system (including detection method and separation buffer), which can be used to detect most samples. Therefore, first use the broad-spectrum detection system to analyze the standard solution series, and then use the separation system of the present invention to analyze the standard solution series.

[0031] After the broad-spectrum detection system was applied to the standard m...

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Abstract

A capillary electrophoresis separation test system applied to soil solution cation composition and concentration thereof. The invention is characterized in that the system contains imidazole of 5-10mM, the concentration of HIBA in a buffer solution is 8-9mM, the concentration of 18-crest-6 is 2-5mM, and the pH value of the test system is 3.5-4.0. The invention aims at providing the capillary electrophoresis separation test system for cation composition in soil in-situ solution. The test system focuses on optimizing the composition of buffer solution to be tested and formulation thereof, thus being widely applicable for fast test of soil solution, in particular for the test requirement featuring complex proportion of composition, unsuitable proportional absorption and higher concentration for partial composition.

Description

1. Technical field [0001] The invention provides a capillary electrophoresis separation detection system, which is especially suitable for the capillary electrophoresis separation detection system of the cation composition and concentration of soil solution. 2. Background technology [0002] The detection technology of cations in soil and rhizosphere solution plays a very important role in the research of soil science, plant nutrition and agricultural environment, and is also an important tool to guide agricultural scientific production. However, for a long time, the detection technology of cations in soil and rhizosphere solution is generally destructively separated to obtain a large amount of rhizosphere soil, then air-dried, and then the soil is leached with different reagents and a certain water-to-soil ratio to obtain the corresponding soil. solution; or after obtaining a large amount of rhizosphere soil, adopt high-speed centrifugation or pressing to obtain fresh soil ...

Claims

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Application Information

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IPC IPC(8): G01N27/447
Inventor 刘新红施卫明
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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