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Virus removal and rapid propagation technology of sweet potato variety 'Shangshu 19'

A sweet potato, detection method technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effects of enhancing epidemic resistance, reducing production costs, and improving quality and yield

Inactive Publication Date: 2011-08-24
周玉玲
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After the sweet potato is infected with the virus, the variety will degenerate, and the yield will drop by more than 30%. In severe cases, the yield of the field will drop by more than 50%, and some even fail to harvest; the sweet potato infected with the virus has rough skin, smaller tubers, and lower dryness rate and starch content.

Method used

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Examples

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Embodiment Construction

[0031] This example is an example of tissue culture and rapid propagation using the nationally approved sweet potato variety "Shangshu 19". This variety is a spring and summer potato type, suitable for a wide range of planting, with a yield of 3000-5000kg / mu, a drying rate of about 33%, and a starch content of more than 30%. After detoxification, the yield increased by 38.9% compared with the control Xushu 18 (detoxification).

[0032] 1. Differentiation and induction culture

[0033] From the growing plants, select plants that are robust, free from diseases and insect pests, and have the characteristics of this variety, cut off the stems with stem tips, remove visible leaves, rinse in running tap water for 20-30mis, and drain the water stand-by. On the ultra-clean workbench in the aseptic room, soak it in 75% alcohol for 20 seconds, sterilize it with 0.1% mercuric chloride solution for 8-12 minutes, rinse it with sterile water 5-7 times, and use 60 -Under an 80-fold dissec...

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PUM

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Abstract

The invention is a virus removal and rapid propagation technology of sweet potato variety 'Shangshu 19', comprising the following steps: selecting plants which are robust without diseases and insect pests and have characteristics of the variety from growing plants; clipping stem with stem tip; removing visible leaves; sterilizing; peeling the stem tip with 1-2 leaf primodia via a dissecting needle; quickly inoculating into a differentiation and induction culture medium; trimming the sterilized seedling into stem segments with 1-2 internode(s) and switching into an enrichment medium after virus detection; selecting sterilized seedling in vigorous growth to cut into small segments with 1-2 internode(s) and switch into a rooting medium; carrying out domestication on seedling after rooting. The invention has stem tip in virus removal and stem segment in rapid propagation, solves the problems of severe virus disease for sweet potato, poor quality of new cultured breed, germplasm degradation, low yield and the like propagated by using sweet potato block and stem. The invention can artificially control the growing conditions, can not be influenced by nature, and has the advantages of small amount of material use, economical culturing materials, short growth cycle, large propagation coefficient and convenient management and is favor of industrial production.

Description

technical field [0001] The present invention relates to a plant tissue culture detoxification and rapid propagation technology, to be precise, it uses the stem tip meristem group of sweet potato for detoxification, stem section asexual tissue culture for rapid propagation, and tissue culture expansion. Nutrients-medium ratio. [0002] Background technique [0003] Sweet potato is a kind of food between grain and vegetable. my country's annual output ranks first in the world. It can be produced in a large-scale intensive manner or cultivated in small-scale gardens. Only Shangqiu City and its surrounding areas plant 1.5 million mu of sweet potatoes all year round. Using sweet potatoes to process starch, vermicelli, and vermicelli after harvest has become an effective way for farmers to get rid of poverty and become rich. However, due to the long history of sweet potato cultivation in our city and its surrounding areas, the damage of sweet potato virus is quite serious. The in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 周玉玲刘广卿吕树立张福娟姜曙光左双喜孙凤岭孙喜云
Owner 周玉玲