Diarrhetic shellfish poisoning okadaic acid OA mouse biological detection model

A kind of technology of haicic acid and detection model, which is applied in the field of marine biology, can solve the problems of organism strain, gender and individual differences, and differences, and achieve the effect of simple and easy operation procedures, stable strains and sufficient quantity

Inactive Publication Date: 2010-01-13
SHANGHAI OCEAN UNIV
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Problems solved by technology

[0005] However, the main disadvantage of the mouse bioassay method is that there are strains, genders, and individual differences in the organism itself, and the test results will vary due to the different mouse strains used.

Method used

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  • Diarrhetic shellfish poisoning okadaic acid OA mouse biological detection model
  • Diarrhetic shellfish poisoning okadaic acid OA mouse biological detection model
  • Diarrhetic shellfish poisoning okadaic acid OA mouse biological detection model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Diarrheal shellfish toxin LD 50 Determination of

[0028] Firstly, the OA standard was diluted with 9 g / L normal saline solution containing 1% Tween 60 to obtain the following 7 toxin concentrations (1 μg / mL, 2 μg / mL, 3 μg / mL, 5 μg / mL, 7 μg / mL, 10 μg / mL, 15 μg / mL). Preliminary tests were carried out to record the poisoning and death of mice, and the minimum total lethal concentration of the toxin was determined to be 7 μg / mL, and the maximum total non-lethal concentration was 2 μg / mL (Table 1).

[0029] Table 1 Toxin lethal concentration results

[0030]

[0031] Set up 8 equal ratio concentration groups within the range of minimum total lethal and maximum total non-lethal toxin concentration, respectively 1.95μg / mL, 2.34μg / mL, 2.81μg / mL, 3.38μg / mL, 4.05μg / mL, 4.86μg / mL, 5.83μg / mL, 7.00μg / mL, conduct a formal test. The symptoms of poisoning and the number of deaths were recorded, and the mortality rate was calculated accordingly (see Table 2). The lo...

Embodiment 2

[0034] Example 2: Determination of toxin dose-intermediate death time curve and toxin dose unit-intermediate death time curve

[0035] In determining LD 50 On the basis of the value, 10 toxin concentration groups were further set up for experiments to establish toxin dose-intermediate death time curves and toxin dose unit-intermediate death time curves. See Table 3 for each concentration group and its corresponding toxin dose, dosage unit and median death time of mice.

[0036] Table 3 dose-intermediate death time test results

[0037]

[0038] According to the data in Table 3, obtain the toxin dose-median death time curve y=600.57x respectively -2.257

[0039] ( figure 1 ) and toxin dose unit-intermediate death time curve y=26.06x -2.257 ( figure 2 ). In the dose range of 5.8-6.5ug, there is a linear relationship between the dose and the death time. At this time, the death range of the mice is 7.20-12.67h. Therefore, in the actual detection work, the death time of ...

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Abstract

The invention relates to an establishment of a diarrhetic shellfish poisoning okadaic acid OA mouse biological detection model, which belongs to the field of detection of marine organisms. The invention uses closed colony Kunming mice, determines the intraperitoneal injection LD50 of diarrhetic shellfish poisoning to China Kunming mice and establishes a dosage-death time curve, a dosage unit-intermediate death time curve and a dosage unit-intermediate death time correlated straight line, thereby laying a foundation for popularization and application of utilizing the Kunming mice for detecting the diarrhetic shellfish poisoning. The use of the detection model for detecting OA has the advantages of strong reliability, low cost and a wide range of applications, the detection model can express the actual content of OA in a sample and does not need complicated equipment; furthermore, the operation procedures are simple and easy to operate.

Description

Technical field: [0001] The invention relates to the establishment of a mouse biological detection model of diarrhea shellfish toxin soft sponge acid OA, which can be used in the technical field of marine biology. Background technique: [0002] Diarrheal shellfish poisoning (DSP) is a class of fat-soluble natural compounds produced by some species of toxic red tide algae Dinophysis and Prorocentrum. The main components are soft spongy acid (OA) and its derivative DTXs. The symptoms of poisoning caused by DSP include diarrhea, nausea, vomiting, and pain in the lower abdomen, which usually occur within a few hours after consumption, or as short as 30 minutes in severe cases, and there is no effective drug treatment. [0003] At present, the methods for detecting OA in China are mainly mouse method, high performance liquid chromatography (HPLC) and immunoassay technology (ELISA). However, most countries in the world still list the mouse method and HPLC as red tide toxin alert ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00
Inventor 何培民柳俊秀
Owner SHANGHAI OCEAN UNIV
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