Unlock instant, AI-driven research and patent intelligence for your innovation.

Liujiaobuxue preparation and fingerprint atlas measuring method of raw material and intermediate thereof

A technology of fingerprints and determination methods, which is applied in the field of determination of fingerprints of traditional Chinese medicines, can solve problems that have not been reported, and achieve the effects of good coincidence and good repeatability of determination

Active Publication Date: 2010-01-13
JIUZHITANG
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many methods for the determination of active ingredients in Astragalus such as astragaloside IV, but how to establish macroscopic fingerprints of chemical components in donkey glue blood-enriching preparations from a more macroscopic point of view has not been reported

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Liujiaobuxue preparation and fingerprint atlas measuring method of raw material and intermediate thereof
  • Liujiaobuxue preparation and fingerprint atlas measuring method of raw material and intermediate thereof
  • Liujiaobuxue preparation and fingerprint atlas measuring method of raw material and intermediate thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Embodiment one (preparation of intermediate, preparation)

[0073] Weigh 216g donkey-hide gelatin, 180g astragalus root, 180g codonopsis 180g, 120g rehmannia glutinosa, 90g atractylodes rhizome, 60g six flavors of angelica, distill angelica and atractylodes rhizome, collect the distillate for later use, decoct the residue with astragalus, codonopsis and rehmannia root three times, add water for the first time 1.5 hours, 1 hour each for the second and third times, combine the decoction, filter, concentrate the filtrate to a clear paste with a relative density of 1.08-1.10 (75-80°C), after cooling, add ethanol to make the alcohol content 50-55 %, stir evenly, cool, stand still, filter, and the filtrate recovers ethanol, concentrates to the extract whose relative density is 1.25, and sets aside.

[0074] Crush donkey-hide gelatin into fine powder, mix with 1800g of sucrose powder, add the above-mentioned extract and distillate, mix well, make granules, dry, and sieve to ...

Embodiment 2

[0075] Embodiment two (preparation of intermediate, preparation)

[0076] Weigh 80g donkey-hide gelatin, 100g astragalus, 100g codonopsis, 80g rehmannia glutinosa, 50g atractylodes macrocephala, and 50g angelica. 2 hours for the first time, 1.5 hours for the second time, combine the decoction, filter, concentrate the filtrate to a clear paste with a relative density of 1.08-1.10 (75-80°C), after cooling, add ethanol to make the alcohol content 50-55 %, stir evenly, cool, stand still, filter, and the filtrate recovers ethanol, concentrates to the extract whose relative density is 1.25, and sets aside.

[0077] Crush donkey-hide gelatin into fine powder, add the above-mentioned extract, distillate and appropriate amount of auxiliary materials, mix well, make granules, dry, and compress into tablets.

Embodiment 3

[0078] Embodiment three (preparation of intermediate, preparation)

[0079] Weigh 200g donkey-hide gelatin, 190g astragalus, 190g codonopsis, 120g rehmannia glutinosa, 90g atractylodes macrocephala, and 70g angelica. 2 hours for the first time, 1.5 hours for the second time, combine the decoction, filter, concentrate the filtrate to a clear paste with a relative density of 1.08-1.10 (75-80°C), after cooling, add ethanol to make the alcohol content 50-55 %, stir evenly, cool, stand still, filter, and the filtrate recovers ethanol, concentrates to the extract whose relative density is 1.25, and sets aside.

[0080] Crush donkey-hide gelatin into fine powder, add the above-mentioned extract, distillate and appropriate amount of auxiliary materials, mix well, make granules, dry, put into capsules, and obtain.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
Login to View More

Abstract

The invention provides a Liujiaobuxue preparation and a fingerprint atlas measuring method of raw material and intermediate thereof; the method comprises the following steps: weighting Liujiaobuxue preparation / raw material / intermediate, extracting by using at least one of water, methanol and ethanol as solvent, performing constant volume process to obtain a sample solution; pipetting the sample solution to inject in a liquid chromatograph, adopting gradient elution through high performance liquid chromatography (HPLC)to measure, and obtaining the fingerprint atlas of Liujiaobuxue preparation / raw material / intermediate; wherein, the chromatographic conditions of the HPLC is as follows: the filler of the HPLC is octadecylsilane chemically bonded silica; the mobile phase A is a mixed solution of acetonitrile and methanol, the mobile phase B is water, the volume ratio of mobile phase A to mobile phase B gradually increases over time; the detection wavelength is 260-275nm. The invention is characterized of simple and stable method, high accuracy, good reproducibility and easy use.

Description

technical field [0001] The invention relates to a method for measuring fingerprints of traditional Chinese medicines, more specifically, relates to a method for measuring chemical components of donkey glue blood-enriching preparations, raw materials and intermediates thereof by high-performance liquid chromatography (HPLC) fingerprints. Background technique [0002] About 3 billion people around the world suffer from varying degrees of anemia, and tens of millions of people die every year due to various diseases caused by anemia. In recent years, with the development of modern life, factors such as environmental air pollution and the increase of chemical synthesis products have made the occurrence of the above diseases have an obvious increasing trend. According to the epidemiological survey, only among 1529 women of childbearing age, the prevalence rate of anemia was 31.2%, while the incidence rate of anemia among rural children in counties (cities) with middle and lower ec...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/74G01N30/34
Inventor 刘春海欧金秀张妮瑜刘淑妦
Owner JIUZHITANG